Storage conditions for Tris hydrochloride (Tris hcl) buffer
Release time:
2023-09-09
Tris hydrochloride, abbreviated as Tris HCl, is a buffer widely used in biochemical and molecular biology experiments. Its main function is to maintain the acidity and alkalinity of the solution, allowing biological molecules to conduct experiments under ideal pH conditions. However, in order to ensure the effectiveness and stability of Tris HCl, correct storage conditions are crucial. Below, we will discuss the storage conditions of Tris HCl buffer and how to greatly extend its shelf life.
1、 Storage conditions
1. Dry environment: Tris HCl powder is very sensitive to humidity, so it should be stored in a dry environment. Wet conditions can cause moisture absorption of the powder and affect its performance.
2. Sealed Storage from Light: Tris HCl should be stored in a sealed container protected from light to prevent chemical reactions caused by light and CO2 in the air. The use of a sealed container can reduce the degradation reaction of Tris HCl powder.
3. Low temperature: When storing Tris HCl buffer, storing it in a low temperature environment can extend its shelf life. The optimal storage temperature is usually between 2 ° C-8 ° C, and its stability is high within this temperature range.
4. Avoid repeated freeze-thaw: Avoid repeated freeze-thaw cycles of powder, as repeated freeze-thaw cycles may cause changes in the form of the powder, such as clumping, which can affect its performance.
5. Regular inspection: Regularly inspect the appearance and properties of the powder. If there are abnormalities such as discoloration, clumping, or odor, it should be considered to replace it with a new Tris-HCl.
2、 Extend the validity period of Tris-HCl
1. Packaging: Large capacity Tris HCl powder can be packaged into small containers to reduce the frequency of opening the container each time, which helps reduce exposure to air.
2. Attention to cleanliness: When using, be sure to pay attention to cleanliness and hygiene, use clean tools and gloves, and avoid introducing impurities into the container.
3. On demand preparation: Do not prepare too much Tris HCl buffer in advance, but prepare it on-site according to the experimental needs, ready to use, which can ensure better buffering effect. Even if the prepared solution needs to be placed, it must be used within 7 days and cannot be left unused for a long time, as it is prone to bacterial growth.
4. Record storage information: Record each use and storage date to track its lifespan and prepare for replacement in advance.
The correct storage conditions ensure the performance and experimental results of the buffer, while avoiding resource waste and effectively saving costs and time. As a production supplier of buffer, Desheng can provide analytical grade Tris HCl powder, which is simple to prepare and can be completed without excessive operation. If you have any interest, please feel free to contact us for purchase!
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