MOPSO， 3- (N-morpholino) -2-hydroxypropanesulfonic acid, also known as 3- (N-morpholino) -2-hydroxypropanesulfonic acid, is a type of biological buffering agent that is widely used in various biochemical and biophysical studies due to its excellent buffering capacity and stability over a wide pH range, especially under neutral to alkaline conditions. In the highly sensitive thermal analysis technique of Isothermal Titration Calorimetry (ITC), the application of MOPSO is mainly reflected in the following aspects:

MOPSO buffer

Maintain sample stability

ITC is a technique for directly measuring the release or absorption of heat during the interaction between two molecules (such as ligands and receptors), in order to calculate thermodynamic parameters such as binding constants and binding enthalpies. During the experiment, the pH value of the sample has a significant impact on its thermodynamic properties. MOPSO, as a buffering agent, can ensure that both ligand and receptor solutions remain in a stable pH environment throughout the titration process, thereby avoiding measurement errors caused by pH fluctuations and ensuring the accuracy and reproducibility of experimental results.

Reduce non-specific adsorption

In ITC experiments, non-specific adsorption may occur between the sample and the surface of the reaction cell, which may interfere with the actual binding heat measurement. MOPSO has a relatively low protein adsorption tendency, and compared to some other buffering agents, it can reduce this non-specific adsorption phenomenon, ensuring that experimental results are purer and closer to the thermal effects of real binding events.

Provide appropriate ion strength

The ITC experiment has some requirements for the ionic strength of the solution, which can affect the measurement of thermal effects if it is too high or too low. MOPSO buffer can adjust the ion strength of the solution by adjusting its concentration, providing a suitable background environment for ITC experiments and facilitating precise measurement of intermolecular interaction heat.

Suitable for specific types of experiments

In ITC studies involving metal ions or pH sensitive biomolecules such as nucleic acids and certain enzymes, MOPSO's non chelating properties make it more favored, especially when it is necessary to avoid coordination interference from metal ions. In addition, the stabilizing effect of MOPSO on proteins is also beneficial for ITC experiments conducted near natural physiological pH.

In summary, the application of MOPSO in temperature drop quantitative calorimetry (ITC) is mainly aimed at creating a stable experimental environment, optimizing experimental conditions by maintaining pH value, controlling ion strength, reducing non-specific adsorption, and ensuring the authenticity and reliability of measurement data. Therefore, MOPSO is not only a biological buffer, but also an indispensable auxiliary agent for precise thermodynamic analysis, especially in research methods such as ITC that require strict experimental conditions.

Product packaging

Desheng Biochemical Technology, as a leading producer of MOPSO buffer, focuses on the research and manufacturing of biological buffering agents. Our MOPSO products achieve the minimization of inter batch differences through refined production processes, ensuring the consistency and reliability of customer experimental results. In order to meet different scientific research and industrial needs, we not only provide MOPSO, but also dozens of other biological buffering agents. Customers can provide trial installation services if they have needs, so that every customer can personally experience the products and performance of Desheng MOPSO before formal procurement, choose with confidence, and help your every research and production project reach new heights.