The pH range of N - (2-acetylamino) -2-iminodiacetic acid (ADA) buffer
N - (2-acetylamino) -2-iminodiacetic acid (ADA) is a multifunctional buffer that not only has superior buffering performance, allowing the solution to maintain a stable pH environment, but also has unparalleled advantages compared to other buffer agents, playing a role in rare fields. Let's take a look together.
1、 PH range of ADA buffer
The uniqueness of ADA buffer lies in its excellent buffering performance in the pH range of 6.0 to 7.2. Within this range, he is able to effectively maintain the acid-base balance of the solution, making the experimental conditions more stable.
2、 Multi domain applications
1. Protein crystallization and microtubule polymerization: ADA is widely used in protein crystallization and microtubule polymerization experiments. Its appropriate pH range enables ADA buffer to provide a stable acid-base environment in these experiments, helping to maintain the stability of proteins or microtubules.
2. Capillary electrophoresis: As a buffer for capillary electrophoresis, it can provide good separation results. Especially when separating amiodarone and deethylamiodarone, ADA exhibits excellent results, which helps to improve the accuracy of the experiment.
3. Differential scanning calorimetry: In differential scanning calorimetry experiments, ADA is used as a sample buffer. Its unique properties allow researchers to delve deeper into the properties of samples such as fibroblast growth factors in situations where phosphate or sulfate buffer solutions cannot be studied.
3、 Precautions and advantages
1. PH measurement accuracy: When using ADA buffer, ensure the use of an accurate pH meter for measurement to ensure the reliability of experimental results.
2. Multifunctionality: The versatility of ADA buffer makes it an important role in many biochemical experiments and can adapt to different experimental requirements.
3. Strong binding of metal ions: In terms of chemical properties, ADA is relatively active and has strong ability to bind metal ions. It can complex with copper, nickel, zinc, calcium, magnesium, manganese, cobalt, etc. It should be noted that the use of ADA buffer is generally not recommended in reaction solutions that require metal cations, and other suitable buffer agents need to be selected based on experiments.
N - (2-acetylamino) -2-iminodiacetic acid (ADA) buffer has received widespread attention due to its unique pH range and versatile applications. Its role in experiments such as protein crystallization, microtubule polymerization, capillary electrophoresis, and differential scanning calorimetry provides researchers with important experimental tools and superior buffering performance.
As a manufacturer of ADA buffer, Desheng can provide raw materials with a purity of over 99%, which is simple to prepare, convenient to store, not easy to deteriorate, and reduces performance. Currently, we have cooperation with 80% of enterprises or laboratories in the market, with up to 100 exporting countries. If you have any interest, please feel free to contact us and give it a try!
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.