Synthesis and configuration of HEPPSO (3- (hydroxyethyl piperazine) -2-hydroxypropanesulfonic acid) buffer
HEPPSO is a biological buffer with good buffering ability and chemical stability, which can maintain acid-base balance of solutions within various pH ranges. Therefore, it is often used in molecular biology and biochemical experiments. The synthesis and configuration of HEPPSO will be introduced below.
Synthesis Method of HEPPSO
Firstly, the synthesis of HEPPSO requires a series of chemical reactions to complete. React hydroxyethyl piperazine with 2-bromoacetic acid. The reaction needs to be carried out under appropriate solvent and temperature conditions, and a certain amount of alkali catalyst is added. Then, the obtained reactant is reacted with propionyl chloride again to obtain HEPPSO product, which is then dried into powder.
Configuration method of HEPPSO
Dissolve a certain amount of HEPPSO in an appropriate solvent, commonly used solvents include water, glycerol, and sodium citrate solution. The concentration of the solution can be adjusted according to experimental needs, and it is generally recommended to be within the range of 10-50mM. During the configuration process, it should be noted that HEPPSO has a low solubility, so sufficient stirring or ultrasonic treatment is needed to increase its solubility.
Precautions for using HEPPSO
1. Select the appropriate HEPPSO concentration based on the required pH range for the experiment. Generally speaking, lower concentrations are suitable for acidic environments, while higher concentrations are suitable for alkaline environments;
2. The buffering capacity of HEPPSO decreases with the increase of temperature, so it is necessary to increase the concentration when used under high temperature conditions;
3. The pH value of HEPPSO buffer solution shifts with temperature changes, so corresponding corrections need to be made according to experimental conditions during use.
Hubei Xindesheng Materials is a manufacturer of biological buffering agents. It was only in 2005 that it was engaged in the production and research and development of buffering agents. It has rich product experience and a wide range of research and development projects. At present, dozens of biological buffering agents have been developed and produced, including TRIS, HEPES, MOPS, CAPS, BICINE, HEPPSO, etc. And the market feedback is good. It can meet the different usage needs of various industries. If you need to make a purchase, please feel free to contact us anytime to inquire and order!
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.