Under what conditions are acridine esters stable and unstable?
Acridine ester is a widely used fluorescent marker in chemiluminescence technology, and its fluorescence signal can provide very high sensitivity, making it an important tool in biological research. The environment of chemical reagents is usually crucial for their performance, and whether they interfere with experiments in different environments is something that most people must understand and master. In which environments are acridine esters stable and which environments are they unstable? Let's take a look together.
1. Acidic environment (pH<4.8): Acridine esters exhibit high stability in acidic environments because their molecular structure is not easily altered under acidic conditions, thus maintaining good luminescent properties.
2. Protein conjugates: After being conjugated with acridine ester and protein, the photoquantum yield does not decrease after being stored at room temperature for 4 weeks, indicating that acridine ester labeled proteins can also maintain stable fluorescence signals under these conditions.
3. Storage of freeze-dried products: In addition, appropriate storage will also ensure its stability, such as freeze-dried products that can be stored at -20 ℃ for more than one year.
1. Alkaline environment: Acridine esters are unstable and prone to decomposition in alkaline environments. Because alkaline environments may cause changes in the molecular structure of acridine esters, thereby affecting their fluorescence performance.
2. Oxidative environment: In addition to alkaline environments, acridine esters also exhibit instability in oxidative environments. Oxidative reactions may disrupt the structure of acridine esters, leading to weakened or even lost fluorescence signals.
3. Lighting conditions: In addition, there is another situation where luminescent markers may decompose under lighting conditions, affecting their fluorescence effect. Therefore, when using acridine ester for fluorescence experiments, strong light exposure should be avoided as much as possible, and light should be avoided during storage.
Acridine esters exhibit good stability in acidic environments, but are less stable under conditions such as alkalinity, oxidation, and light exposure. Therefore, when conducting acridine ester fluorescence experiments, attention should be paid to the acidity and alkalinity of the environment, avoiding alkaline and oxidative conditions, as well as excessive light exposure. This will help maintain the good fluorescence performance of acridine ester, thereby obtaining accurate and reliable experimental results.
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