Luminescence characteristics of acridine ester NSP-SA-NHS 199293-83-9
Acridine ester is a highly regarded chemical luminescent reagent that is not only favored by customers, but also has excellent performance. At present, most manufacturers, in order to facilitate transportation and storage, produce acridine ester NSP-SA-NHS in yellow powder form. When used, it is prepared into a solution. After contact with hydrogen peroxide, it usually does not require enzyme catalysis to emit light, making it suitable for various luminescence analysis experiments. What are its specific luminescent characteristics? Below is a specific introduction for everyone.
1. Luminescence wavelength: under the attack of hydrogen peroxide ion, it can generate unstable dioxane, and then decompose into carbon dioxide and acridone in excited form. When it returns to the ground state again, it can emit photons with an absorption wavelength of 430 nm. This wavelength makes the luminescence process fast, ending in about 2 seconds, achieving the goal of rapid luminescence while also being absorbed and emitted by most biological molecules.
2. Luminous intensity: Its luminous intensity generally varies based on the concentration and pH value of the test sample. If the environmental conditions of the test sample are the same, the peak intensity can be reached within 0.4 seconds after adding the excitation solution, and the half-life is about 0.9 seconds.
3. Stability: Acridine ester NSP-SA-NHS has good stability and will not lose its luminescent properties after being sealed and stored in a dark place at room temperature for a period of time. But it is also important to pay attention to the surrounding storage temperature. It is recommended to refrigerate the reagent in the refrigerator, check the validity period of the reagent before use, and prepare it for use in a timely manner.
4. Sensitivity: It has high sensitivity and does not require catalysts or enhancers in experimental reactions. It only needs to be tested in a dilute alkaline solution with H2O2 to emit light, and its structure is simple and easy to undergo chemical reactions.
5. Wide application range: It is suitable for labeling various biological molecules, such as proteins, nucleic acids, sugars, etc. At the same time, due to its sensitivity and stability, it has also been applied in research such as fluorescent probes, receptor analysis, and peptide detection.
The luminescent characteristics of acridine ester represent its quality and also indicate the effectiveness of the experiment. Therefore, it is important to ensure that the purchased product has no problems before use. Desheng is a professional supplier of luminescent reagents. The acridine ester series of products are analytical grade, with pure appearance and color, uniform powder, and easy preparation operation. It is one of the manufacturers that most people are willing to choose. If you are also interested, please click on the website to inquire about the details!
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