How to prepare and store Luminol solution?
Luminol solution is a commonly used chemical reagent, often used in fields such as biochemistry and medical analysis. In order to ensure the accuracy and safety of the experiment, it is crucial to correctly prepare and preserve Luminol solution. The following will introduce the preparation and preservation methods of Luminol solution from the following aspects.
1、 Preparation method
1. Prepare tool materials: balance, measuring cup, stirring rod, sodium hydroxide, luminol, potassium ferrocyanide and distilled water, hydrogen peroxide.
2. Accurate weighing: When preparing, it is necessary to use a balance to accurately weigh the mass of each chemical to ensure the accuracy of solution concentration, such as accurately weighing 0.2g of sodium hydroxide, 0.1g of luminol, 0.3g of potassium ferrocyanide, 100ml of distilled water, etc.
3. Operation steps: Pour distilled water into a measuring cup, add sodium hydroxide and luminol, drop in hydrogen peroxide, and continue stirring until the powder is completely dissolved. Count the solution as reagent A. Then take potassium ferrocyanide, inject it into distilled water, and continuously stir until it is completely dissolved. Count the solution as reagent B, and then dilute the required concentration by mixing reagent A with reagent B.
2、 Saving method
1. Storage environment
Luminol solution should be stored in a cool, dry, and well ventilated place, avoiding direct sunlight and high temperatures, while also keeping away from harmful substances such as ignition sources and oxidants.
2. Avoid vibration and shaking
During storage and transportation, severe vibration and shaking should be avoided to prevent chemical reactions or precipitation in the solution. Shockproof packaging or cushioning materials can be used to protect the solution from vibration.
3. Label annotation
When storing Luminol solution, labels should be affixed to the container, indicating the solution name, concentration, preparation date, and other information, which can facilitate future use and management.
1. Pay attention to safe operation
When preparing and using Luminol solution, attention should be paid to operational safety, protective equipment should be worn, and direct contact with skin and eyes should be avoided. If it accidentally comes into contact with skin or eyes, it should be immediately rinsed with plenty of water.
2. Storage time
Luminol solution should be used as soon as possible after preparation, and generally should not exceed 24 hours. If it needs to be stored for a long time, it can be diluted to a certain concentration and sealed in the refrigerator. However, it should be noted that the diluted solution may lose some of its active ingredients, so it needs to be prepared again before use.
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