There are many principles of in vitro diagnostic reagents. For example, enzyme-linked ImmunoSorbent Assay (ELISA), enzymatic Assay, colloidal gold Assay, immunoblotting method and so on.
Each principle can be applied to the detection of multiple items to be tested. Based on the principle of Trinder reaction (also called enzymatic method), it can be used to detect uric acid, creatinine, blood glucose, cholesterol, triglyceride, etc. So what is Trinder's reaction and Trinder's reagent.
Trinder 's response:
This reaction was first proposed by Trinder in 1969, hence the name Trinder reaction. The principle is that the hydrogen peroxide (H2O2) produced by the enzyme action of the measured substance makes the chromogen material produce red quinone imine compound in the presence of 4-aminotipyrine (4-aap) and peroxidase (POD). Initially, phenol was used as a chromogenic agent. Later, many compounds were substituted for phenol, which significantly improved the sensitivity of the reaction. The Trinder reaction forms the basis of enzymatic determination of glucose, cholesterol, triglyceride and uric acid.
Trinder 's reagents:
The chromogenic substance or chromogenic agent referred to in the Trinder's reaction is the Trinder's reagent, also known as the chromogenic substrate or hydrogen donor. Traditional Trinder's reagents: phenols: phenol, 4-chlorophenol or 3, 5-dichloro-2-hydroxybenzenesulfonic acid sodium, etc.
Aniline: N, n-dialkyl aniline,N, n-dialkyl - m-toluidine, etc. New Trinder's reagent:
Aniline sulfonate (10) : ADPS, TOOS, TOPS, ADOS, HDAOS, DAOS, ALPS, MAOS, MADB, TODB.
Disadvantages of traditional Trinder's reagent
Disadvantages of phenolic reagents:
The maximum absorption wavelength of the product of the color reaction formed by the Trinder's reagent is 500~520nm, while the absorption wavelength of bilirubin in the serum is 380~530nm, which will interfere with the detection results. It is necessary to add potassium ferrate cyanide to eliminate the interference.
Disadvantages of aniline reagents:
The maximum absorption wavelength of the product of the color reaction formed by the Trinder's reagent is 550~555nm, but the color reaction can only be carried out under acidic conditions, and many enzymes will be inactivated under acidic conditions, which greatly limits the application scope.
1. High water solubility
2. Uv absorption of color reaction product > 540nm
3. The color reaction requires a wide PH range
4. High color sensitivity.
DE sheng, the current development of product of new-style Trinder reagent, the advantage is: high purity products, process stability, can guarantee to provide products, white light, these three indicators have had a very strong market competitiveness, in ensuring the quality of products at the same time, the spontaneous development of production mode will give a more populist price to customers, help customers save middlemen, saving purchasing cost at the same time, DE sheng will continue strong, produce more high-quality reagent products.