Application of MADB chromogenic reagent

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MADB chromogenic reagent is one of Trinder's reagent: chromogenic substance or chromogenic agent in Trinder's reaction is Trinder's reagent, also called chromogenic substrate or hydrogen donor.

Trinder 's response:

This reaction was first proposed by Trinder in 1969, hence the name Trinder reaction. The principle is that under the presence of 4-aminopyrine (4-aap) and peroxidase (POD), the H2O2 produced by the measured substance through enzyme action can generate red quinone imine compounds. The initial use of phenol as chromogenic agent was replaced by a number of compounds, which greatly improved the sensitivity of the reaction. Trinder reactions form the basis of enzymatic determination of glucose, cholesterol, triglycerides and uric acid.


There are many detection principles of MADB chromogenic reagent. Enzyme-Linked ImmunoSorbent Assay (ELISA), colloid gold Assay, western blot Assay, etc. Each principle can be applied to the detection of multiple items to be tested. For example, Trinder reaction (also called enzyme method) can be used to detect uric acid, creatinine, blood glucose, cholesterol, triglyceride and so on in blood test.

MADB chromogenic reagent can also be called MADB chromogenic substrate,

Butyl and dimethylaniline disodium salt and butyl and dimethylaniline disodium salt and butyl and dimethylaniline disodium salt

[Chinese name] N, n-bis (4-sulfobutyl) -3, 5-dimethylaniline sodium salt

[purpose] it can be used as a water-soluble reagent for the determination of hydrogen peroxide by enzyme spectrophotometry, and is widely used in diagnostic detection and biochemical tests

[product advantages] purity: >99%, good water solubility, stable process, can ensure the appearance of the product is pure white crystal powder.


Many enterprises will encounter many problems when purchasing MADB chromogenic reagent, what should be paid attention to? Purchased reagent color should be colorless or light, preferably colorless or white, otherwise the color of the material itself will interfere with the observation of the color reaction.


Desheng is an old blood reagent company with 14 years' r&d and production experience. It has deep research on Trinder's reagent and professional advantages on chromogenic substrate MADB,TOOS, MAOS and other new chromogenic reagent. Desheng technology is determined to become the world's leading brand in this industry and make contributions to human health. At present, the company has reached a long-term relationship with many large enterprises.