The configuration method and application of CHES buffer

Release time:


CHES buffer, Chinese name 2-cyclohexylaminoethanesulfonic acid, is a dipolar ionic (zwitterionic) Good's buffer used at relatively high pH values ​​(up to 10). It can be used in several areas of biological research including enzymology, but also to study reactions above the physiological pH range, protein stabilization, mannanase activity assays, DNA digestion and 2'-deoxyadenosine 3'- Monophosphate labeled and used as a component of Wilson and Horne extraction buffer. In addition, it is also very common in electrophoresis experiments, and it has low reactivity with most metal ions. This shows its versatility in various biochemical, molecular biology and molecular diagnostic applications.

CHES buffer has a PKA value of about 9.3 at 25 °C, and the powder has good water solubility. 15 grams of CHES powder can be dissolved in 85 ml of water. Sodium hydroxide can be used to adjust the pH value, because it is acidic in water, so Sodium hydroxide is required to adjust the pH to 8.6-10.

The experimental configuration method is as follows:

(1) Weigh 1.036 g of CHES (MW=207.3 g/mol) and add 40 mL of pure water;

(2) The pH value of 40 mL of CHES aqueous solution was measured to be 4.50, and the test temperature was 27 °C;

(3) After adding 1 mL of 1 M NaOH solution, the tested pH value is 8.83;

(4) Add purified water to 50 mL, transfer to a 50 mL sample bottle, and store at room temperature.

According to the above method, the CHES buffer solution mother solution with pH of about 9.0 can be prepared. During the experiment, the final pH is about 8.83, but there will be a little deviation, which is close to 9.0, which meets the experimental requirements. In order to obtain reliable data during the experiment, the experimental details are very important when configuring, so the basic knowledge required in the experiment needs to be taken seriously.

Desheng Company is a manufacturer of biological buffers. The CHES buffer currently produced is in the form of white powder with a purity of up to 99% and good batch stability. More product information, you can also call to order directly, looking forward to your letter!