Three common problems in the application of chromogenic substrate ADOS

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The chromogenic substrate ADOS is a raw material for color development in the biochemical kit, CAS number: 82692-96-4, which can be oxidized by hydrogen peroxide in the presence of peroxidase to produce color with 4-AAP The color reaction is sensitive and the detection is rapid. The instructions for using the ADOS reagent produced by our company are provided here for reference only.


What should I do if the ice pack melts after receiving the goods?

After receiving the goods, the built-in ice pack has melted. Will the product deteriorate? This product is stable at room temperature. Low temperature transportation is to prevent extreme temperature conditions that may occur during transportation. Therefore, when you receive the product, if the ice pack has melted, it will not affect its quality, please rest assured to use it. If it is stored for a long time, it is recommended to store it in the cold and avoid light. If it is to be prepared into a solution, it is recommended to use it immediately to avoid oxidation and discoloration in contact with air.


What is the specific detection method of ADOS?

1. Prepare sample solutions for enzymatic oxidation reactions. The pH range of the buffer should be 5.5-9.5.

2. Use the same buffer to prepare a standard solution containing a known amount of substrate.

3. Add an appropriate unit of oxidase to the sample solution, and then add an equal volume of the detection solution.

4. Incubate the mixture at room temperature or 37°C for 30 minutes to 1 hour.

5. Prepare a standard curve and determine the concentration of the substrate in the sample solution.



ADOS reagent packaging and precautions for use:

Desheng’s ADOS reagent packaging box is a foam insulation box, which contains an ice bag, and contains a product manual and test report. If it is not complete, please call customer service to request an electronic version. This product needs to be sealed and stored in a dry and cool place. It needs to be packaged in a dark brown bottle with better protection from light. ADOS is a white crystalline powder. If the color darkens, it may have bacteria or partial oxidation and cannot be used. ADOS powder may adhere to the wall of the tube or bottle when it is dissolved. It is allowed to use a centrifuge at low speed to reduce product loss; the product has good solubility and is directly dissolved in deionized water; double steam can be used for higher experimental requirements Water or ultrapure water.


Desheng has 15 years of research experience in research and development and production of chromogenic substrates, with mature technology and team, the purity of the ADOS products produced is greater than 99%, product packaging specifications, ensure transportation safety, timely after-sales service, and ensure customer satisfaction The product.