Which is better, TAPS or BICINE?
Release time:
2023-09-16
In the research of biochemistry and molecular biology, selecting appropriate buffering agents is crucial for maintaining the stability of the experimental system and ensuring the accuracy of the results. But buffering agents are a "big family" with different categories, and their performance and corresponding fields of use vary. Recently, it was discovered that someone was searching online for buffering agents TAPS or BICINE, which one is better? So let's provide a detailed explanation of these two different buffering agents to help you determine your choice under different experimental conditions.
1、 BICINE buffer
BICINE (N, N-di (hydroxyethyl) glycine) is a zwitterionic buffer with a Pka value of approximately 8.3 at 25 ℃. BICINE buffer is suitable for experiments with pH values ranging from 7.6 to 9.0, with a working concentration of 3-100mM, making it particularly suitable for low-temperature biochemical experiments.
BICINE has many advantages, one of which is that it does not react with calcium and magnesium ions in biological systems, making it an ideal choice for many biochemical experiments. In addition, it can effectively chelate heavy metal ions such as copper, cadmium, and lead, and is often used to repair soil contaminated by heavy metals. Due to its enzymatic properties, it has become an effective buffer for protein crystallization solutions, chromatography, and polymerase chain reactions.
2、 TAPS buffer
TAPS (tri (hydroxymethyl) methyl-3-aminopropane sulfonic acid), with a pH buffer range of 7.7-9.1, is suitable for various biochemical and molecular biology experiments. TAPS is often used as a buffer component for DNA and RNA samples, and its advantage is that it can protect oxygenated hemoglobin in a freeze-drying environment, prevent it from undergoing oxidative degradation reaction and forming methemoglobin. In addition, TAPS is also excellent in background electrolytes, commonly used in protein microanalysis, and as an important pH stabilizer, suitable for biochemical diagnostic kits.
3、 Selection of TAPS and BICINE
TAPS and BICINE are both very useful buffers in biochemical and molecular biology research. Due to their different performance, specific experimental requirements need to be considered when selecting them. For example, if your experiment needs to be conducted at a lower temperature or to combat heavy metal pollution, BICINE may be a better choice. If your experiment needs to be conducted in the pH range of 7.7 to 9.1, or involves the processing of DNA and RNA samples, TAPS may be more suitable. The selection of buffering agents must depend on your experimental conditions, and at the same time, it is important to carefully consider parameters such as temperature and ion strength, and verify and optimize them based on specific experimental conditions. It is important to keep this in mind.
As a manufacturer of buffering agents, Desheng provides TAPS and BICINE as analytical grade raw materials. High purity means no impurity interference and strong experimental stability. At the same time, the company can provide customized services and spot sales. If you are interested, please contact us to purchase!
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2023/09/27
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