Advantages and Disadvantages of Acridine Esters in Chemiluminescence Immunoassay

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Chemiluminescence immunoassay refers to the use of acridine ester and other chemiluminescent reagents to directly label antigens or antibodies, and the addition of luminescent enhancers for luminescence reactions. Quantitative or qualitative detection of luminescence intensity is carried out through this detection method, which is often used for in vitro diagnosis. There are similar and equivalent detection methods, such as Radioimmunoassay and enzyme-linked Immunoassay. How does chemical Immunoassay stand out among many detection methods?

Acridine ester

First of all, compared with Radioimmunoassay, chemiluminescence immunoassay has the same sensitivity and will not cause pollution. The advantages of using acridine ester to label antibodies are mild labeling conditions, no damage to antibody activity, and less amount of antibodies used in labeling; Secondly, compared with enzyme-linked Immunoassay, chemiluminescence immunoassay is simpler with the same sensitivity. It does not require biotin and avidin reactions, nor does it require enzyme substrate color development, and the amount of antibodies is also less.

All detection methods have advantages and disadvantages. The disadvantage of chemiluminescence Immunoassay is that the antibody is labeled with acridine ester, the storage period of the marker is short (only one month), and the luminous intensity is not stable. With the continuous progress and development of technology, relevant studies have shown that glycerol has a certain protective effect, which can extend the shelf life of antibodies in epidermal growth factor luminescent immunoassay to six months; The use of detergents not only enhances the luminous intensity, but also helps to maintain the stability of the measurement system.

Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of chemiluminescent reagent acridine ester, and has developed six types of acridine ester products, including DMAE-NHS and NSP-DMAE-NHS. The products are all light yellow powder, with good water solubility, high sensitivity, stable production process, and small batch differences. The company has a large amount of stock, fast delivery speed, and can accept customized services. If you have the need to purchase acridine ester and other chemiluminescent reagents, you can click on the Desheng official website to learn more details or contact me!