Introduction of the Enzyme Immunoassay Substrate Reagent DAOS
The enzyme linked immunosorbent substrate reagent is a very important reagent, which is widely used in the fields of biomedicine and molecular biology. In the experiment, we often use DAOS enzyme linked immunostaining substrate reagent, which is called N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-di Methoxy group aniline sodium salt in Chinese, CAS No. 83777-30-4. This reagent has the characteristics of small molecular weight and high sensitivity.
DAOS color reaction
1. Application of DAOS reagents
DAOS is widely used in various immune detection methods, such as ELISA, immunoblotting, immunohistochemistry, etc. It has been adopted by many research institutions and hospital laboratories, and can be used for protein detection in various biological samples, such as blood, urine, cerebrospinal fluid, etc. The use of DAOS reagent is extremely convenient, and fluorescence detection can be carried out by adding DAOS reagent and hydrogen peroxide substrate. DAOS reagents have high detection sensitivity and can detect extremely small protein concentrations, making them widely used in many biomedical and molecular biology studies.
2. Characteristics of DAOS reagents
As a relatively new chromogenic substrate reagent, DAOS reagent mainly converts the substrate into fluorescent products through Enzyme catalysis, and quantitatively analyzes the fluorescent products by fluorescence spectrometry in the detection process. DAOS reagent is different from other substrate reagents in that it has excellent durability and is not easily affected by environmental factors, thus ensuring the stability and accuracy of the detection results.
Desheng is a manufacturer of color developing substrates. Currently, there are more than ten types of color developing reagents available, including TOOS, TOPS, ALPS, HDAOS, DAOS, and other options. The purity of the product can reach over 99%, with small inter batch differences and stable performance. Professional personnel are available for reception before, during, and after sales, providing technical support. If you need to make a purchase, please feel free to call us for inquiries and orders at any time!
In chemiluminescence analysis, the luminescence intensity of acridine ester is influenced by various factors, such as reaction medium, temperature, time, and excitation light source energy. To achieve good detection results, it is necessary to comprehensively consider and optimize these factors. Meanwhile, attention should be paid to controlling and standardizing experimental conditions to ensure accurate and reliable results. Thoroughly studying these influencing factors will help promote the development of chemiluminescence analysis methods.