Five important indicators for selecting biological buffering agents in experimental projects
Biological buffering agents are essential reagents and consumables in the laboratory. They usually exist in some biochemical experiments to regulate pH values, and are an important step in the research and development process. Therefore, selecting a suitable biological buffering agent for the experiment is the key to determining the success or failure of the experiment. This article will teach you 5 important indicators for selecting biological buffes, which I believe will be helpful for your experiment.
1. Pka value of biological buffer
Choose a pH range suitable for your experimental range according to the experimental needs. If the pH value will decrease or increase during the experiment, choosing a buffer with a pH value higher or lower than the range value before the experiment is an important factor in determining the success or failure of the experiment. Each biological buffer has a different pH range, and the pH range of most biological reactions is around 6-8. You can choose the appropriate one according to your own needs.
2. Water solubility of powder
The solubility of different buffering agents also varies, usually with high solubility in water, while buffering agents with low solubility in organic solvents are more favored by experimenters. Because the better the water solubility of the buffer, the easier it is to prepare a concentrated stock solution. But it is important to note that once the concentrated original solution is diluted, the pH value may change.
3. Salt effect
Many biological systems are affected by salt, so a high salt effect in buffering agents can affect experimental results. However, in some experiments, salt needs to be added to improve the buffering ability of the compound, so there is no need to worry too much about salt effects.
When choosing a biological buffer, permeation is a key factor, and the selected buffer cannot penetrate any cell membrane. If a buffer penetrates cells, it can alter them. This may change the research results.
5. Ionic strength
Ionic strength is a factor that needs to be carefully considered. The biological system may have serious complications due to the high ionic strength in the buffer solution, which may affect the experimental results.
When choosing a biological buffer, it is important to consider these factors. We also need to choose a company that can provide effective and high-quality buffer solutions. Desheng can provide a variety of high-quality biological buffer solutions such as TRIS, HEPES, BICINE, MOPS, etc. You are welcome to contact us anytime if you need to purchase them!
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