How does acridine ester label nucleic acids? This method is very important
Acridine ester, as an important luminescent reagent, plays an undeniable role in both medical and biochemical research and analysis fields, especially in nucleic acid labeling, surpassing other luminescent reagents. Nucleic acid labeling is a crucial step in in vitro diagnosis. Generally, preparing highly specific and sensitive markers can effectively assist in labeling and even ensure the success of experimental detection. And acridine ester can be quickly labeled on nucleic acid probe instruments, and can be detected through luminescence without any catalyst. So how does it specifically label nucleic acids? Below, we will focus on introducing the methods.
1、 The importance of nucleic acid
As an important substance in biological molecules, it is widely present in animal and plant cells and microorganisms. In addition, in medical research, many experiments have shown that major diseases, such as cancer, tumors, or genetic diseases, are related to nucleic acid mutations. In addition, most infectious diseases are also caused by nucleic acids present in bacteria or viruses in the environment, so nucleic acid labeling is an essential research step in medicine and even major biochemical experiments, which is also conducive to disease discovery and control.
2、 Acridine ester labeled nucleic acid
In nucleic acid analysis, hybridization analysis is the key point, and researchers can quickly determine using highly sensitive and responsive luminescent reagents. At the same time, because it is directly labeled on the probe and does not require the assistance of other reagents, it can greatly avoid external interference and complete the entire nucleic acid hybridization analysis experiment without any separation.
3、 Labeling nucleic acid methods
This labeling method mainly involves three key steps: first, protecting nucleic acid probes at different ports; Then, the acridine ester is labeled on the probe. During the labeling process, an appropriate amount of acridine ester needs to be dissolved in methyl sulfoxide and a HEPES buffer with a pH value of 8 is prepared; Add it to the buffer solution under the condition of 1:5 and react at 37 ° C for about 1 hour. Then, probe according to the molar ratio, and purify and separate the nucleic acid using HPLC after labeling.
As a professional manufacturer of chemiluminescence reagents, Desheng offers a full range of products, including 6 types of acridine esters and 3 types of luminol. Based on different customer needs for experiments, we can choose products that are suitable for our customers, with stable luminescence and worry free use. Welcome interested parties to click on the website for consultation and purchase!
Tris, as the "leader" of the buffering agent family, ranks first in both customer registration and popular buffering agents in the market. Its raw material price and quality have become a focus of attention. As one of the suppliers of trimethylaminomethane, Desheng has been favored by a large number of customers due to its cheap raw materials.