Don't be foolishly confused about Tris ethanesulfonic acid and Tris propionic acid anymore
Release time:
2023-04-28
There are many members in the biological buffer family, including Tris, Bicine, Caps, etc. These small members can also derive other buffer systems, such as Tris hydrochloric acid, Tris acetate, Tris glycine, etc. They are all composed of Tris and acids, but sometimes a few manufacturers use organic synthesis methods to combine Tris with some acids to form new molecules, such as Tris ethanesulfonic acid and Tris propionic acid. These two types of buffer solutions are often confused by many people due to their similar names, but in fact, they have fundamental differences. Below is a specific introduction for everyone.
1、 Tris ethanesulfonic acid
It also refers to TES, with CAS number 7365-44-8, molecular weight 229.25, solubility in water 50g/L, and a structure similar to trimethylaminomethane. It is a newly developed physiological buffer of ethanesulfonic acid series, which meets the standards of Good's buffer.
Although its structure is neutral, it behaves as zwitterion in solution, with pKa value of 7.4. Based on its performance, it is widely used in cell culture system of metal ions, while other types of buffer solutions will produce precipitation, which cannot meet this requirement.
In addition, sulfonic acid buffer solutions are generally not recommended for high-temperature sterilization treatment, and attention should be paid to using 0.22 during use μ M's filtration membrane is used for sterilization, and during transportation and storage, to ensure the stability of the solution, it should be stored in an environment of 2-8 degrees Celsius.
2、 Tris propionic acid
Tris propionic acid, as a "general" in the type of buffer, is a zwitterion buffer widely used in the field of biochemical experiments. The pH buffer range is 7.7-9.1, and the water solubility is 25g/50ml. In clinical diagnosis, it can be used as a buffer in biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits. Compared with TES, its purchase and usage rates are relatively high, but it is also important to note that it is ready to use and should not be left unused for a long time, as it is prone to bacterial growth.
The above two types of buffer solutions are not easily distinguishable, so we will introduce them separately. We hope that you can correctly identify them during use or purchase in the future. As a professional supplier of buffer solutions, Desheng has a purity of over 99% for all buffer solutions. The process is stable, convenient and fast to use, without doping with other unknown substances, and the experimental results are good. If you have any purchasing intentions, please click on the website to inquire about the details!
News
2023/09/27
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