Why is Tris testing ineffective? One article to resolve doubts

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Tris, as a member of the biological buffering agent family, usually plays a role in regulating pH values and ensuring the stability of the experimental environment. However, sometimes experimental results may not be satisfactory. Why is this? In fact, it is closely related to the performance of Tris itself, but many people do not know how to distinguish whether the performance meets the standard when purchasing, or conduct performance tests on Tris, but still do not find any abnormalities. Based on this situation, the following will focus on introducing the precise measurement steps to everyone.

1、 Appearance measurement

In fact, appearance testing is relatively simple. Under normal light, visual inspection of Tris powder to see if it contains impurities is sufficient.

2、 Determination of solubility

Weigh a certain amount of Tris powder with a balance and pour it into a conical flask. Add pure water and stir to melt. After dissolution is complete, visually observe again for any residual powder that cannot be dissolved or if the liquid is transparent and clean.

3、 PH measurement

Prepare the pH measuring instrument, preheat it in advance, adjust the parameters, place it in the prepared tris solution, gently shake it, and wait for the solution to stabilize before reading.

4、 Melting point determination

Prepare a micro melting point meter and capillary, insert one end of the capillary opening into the tris powder for inversion, so that the powder falls into the bottom of the tube. Gently tap until the sample is densely packed at the bottom of the tube. Then set the temperature and rate of the melting point instrument. After the set temperature stabilizes, insert a capillary tube and observe the measurement results.

5、 Iron ion determination

Prepare standard extraction solution and dilute hydrochloric acid, sample powder, dissolve with water, transfer to a colorimetric tube, add dilute hydrochloric acid and ammonium persulfate, then dilute with water to volume, then add ammonium thiocyanate solution and water for dilution, shake well for determination.

6、 UV absorbance measurement

Use ultraviolet spectrophotometer to measure, accurately weigh part of the sample, pour it into a volumetric flask, add purified water to dissolve it to a constant volume, after preparation, measure different cuvettes at different wavelengths with a photometer, and record the measured absorbance results.

Desheng is a professional supplier of Tris, which has undergone professional testing in both appearance and performance, and can provide customers with testing reports. Collaborators who have purchased Tris in the market have all given positive feedback, which does not interfere with the experimental process and can be boldly used. If you are interested, please call to inquire about the details!