What are the reasons for the abnormal increase of acridine ester luminescence value?
Release time:
2023-03-21
At present, there are roughly three kinds of chemiluminescence reagent products popular in the Chinese market, namely tripyridine ruthenium, acridine ester and HRP and ALP (both HRP and ALP are enzymatic luminescence), and acridine ester is a popular main product as a direct chemiluminescence reagent.
There are many derivatives of acridine ester, such as NSP-DMAE-NHS, NSP-SA-NHS, DMAE-NHS and NSP-SA, which are widely used in various fields because of their luminous speed, luminous value and other characteristics, but sometimes acridine ester's luminous value is abnormally high. What is the reason? Let's give you a specific analysis so that we can solve the problem in the later stage.
1、 Cuvette static electricity
In some cases, static electricity may occur during the transportation and storage of the cuvette produced by the manufacturer. Acridine ester is used for detection. First, a certain concentration of sodium hydroxide solution is injected into the cuvette, and then the reading operation is performed. However, during solution injection, static electricity in the reaction cup may be released and captured by the radio multiplier tube, resulting in an increase in the luminous value.
2、 Acridine ester pollution
This situation usually occurs in the preparation stage, because the acridine ester used by many people is in powder form and needs to be prepared into a solution. During this period, a small amount of powder may be scattered in the surrounding environment and exist in the air. If the content of acridine ester powder in the air reaches a certain level, it will pollute the open instruments, which is also a factor leading to the increase of the luminous value, which needs to be paid attention to.
3、 The reaction system is not optimized
When acridine ester is used in antigen-antibody, the magnetic beads may gather during the immune reaction in the chemiluminescence system of various manufacturers, which will lead to abnormal experimental results and luminescence values of acridine ester. However, most of the fully automated analytical instruments are closed systems, and it is difficult to detect the phenomenon of magnetic bead aggregation, which is also the key that many operators are easy to ignore. At this time, the method adopted is to optimize, such as optimizing pH value, salt concentration or magnetic beads.
As the R&D and manufacturer of luminescent reagents, Desheng has been pursuing reagent products with high sensitivity, high luminous rate and stable performance, and has continuously invested a lot of time and money to serve the market and customers. At present, the luminescent reagents like acridine ester and luminol series have been sold all over the world, and are the preferred manufacturers of the public. If you are interested, please click the website to purchase!
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