Will the anticoagulant in the vacuum blood collection vessel fail?

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The addition of anticoagulant in the vacuum blood collection tube can effectively prevent blood coagulation, so it is widely used in medical and biochemical experiments. The commonly used anticoagulants include EDTA potassium salt, heparin salt and sodium citrate. Since it is one of the commonly used reagents, will it fail when added to the vacuum tube? In fact, anticoagulants will fail, but this is related to some factors and cannot be generalized. Let's give you a detailed analysis.

1、 Storage time of anticoagulant tube after blood collection
When we conduct blood testing, we often use anticoagulant tubes, which play a major role in preventing blood coagulation within a certain period of time, so as to facilitate the detection of medical staff. When the blood is drawn to the test tube, there is usually a storage time. If the time limit is exceeded, the anticoagulant effect will naturally fail. In the routine blood test, it can be stored for about one week under the condition of 2-8 ℃. If it is a biochemical test, it will not exceed 12 hours.

2、 Other factors leading to the failure of anticoagulant
1. The blood itself is thick, the fluidity is not good, and it does not fully contact with the anticoagulant, resulting in blood coagulation is considered as anticoagulant failure.

3. The amount of anticoagulant is related to the blood content. If the amount of anticoagulant is not enough, it will also lead to failure.

4. The blood sample and anticoagulant are not completely mixed, and the medical staff forget to slowly reverse the operation after blood pumping, which will make the anticoagulant ineffective or ineffective.

5. Anticoagulant tubes need to be used correctly. A few experimental items do not need anticoagulation, but most of the items need anticoagulation. At this time, it is necessary to distinguish and select anticoagulants for appropriate test items. For example, anticoagulants containing potassium salt and sodium salt cannot be used for the determination of potassium ion or sodium ion, and anticoagulants containing EDTA cannot be used for the determination of calcium ion samples. If the anticoagulants are used incorrectly, the direct consequence is the deviation and failure of the detection.

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