Introduction and usage of biological buffer HEPPSO sodium salt
You must be familiar with biological buffers. It is an organic substance that neutralizes hydrogen ions. Biological buffers help maintain the pH value within a certain range, so that the biochemical and experimental processes can continue to be It operates in normal state and is not affected by other factors. There are dozens of types of biological buffers, most of which are composed of weak acids and weak bases. Today, let's take a look at HEPPSO sodium salt.
Introduction of HEPPSO sodium salt:
HEPPSO sodium salt Its Chinese name is N-(2-hydroxyethyl)piperazine-N'-(2-hydroxypropanesulfonic acid) sodium salt, because its chemical name is too long to remember, so HEPPSO sodium salt is commonly used Abbreviation. Its CAS number is 89648-37-3, the pH range is: 7.1-8.5, and the pka value is 7.8 at a temperature of 25 degrees Celsius.
It can be used as an effective buffer for pH adjustment in biochemical experiments and as an excellent separator in IEF protein separation experiments. However, the appearance of HEPPSO sodium salt is white powder before it is prepared, so it needs to be prepared with water and sodium hydroxide or potassium hydroxide.
The configuration method of HEPPSO sodium salt:
(1) According to the requirements of the experiment, take a quantitative HEPPSO sodium salt powder and dissolve it in deionized water;
(2) Titrate to the desired pH value with sodium hydroxide or potassium hydroxide;
(3) Fill to final volume with deionized water;
(4) After high pressure sterilization, it can be stored below 4 degrees Celsius.
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