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New Trinder's reagent DAOS, a mysterious member of those testing programs

Release time:

2022-03-21

With the improvement of material living standards, people's bodies have many different problems due to the accumulation of various high protein and high fats. The common three highs, cardiovascular and cerebrovascular diseases are not uncommon. So how can we manage and prevent these diseases from happening ? This starts with our various routine tests. When it comes to testing, it is inseparable from the various raw materials in the testing. The detection principle I will show you today is DAOS, a member of the new Trinder's reagents that we are not very familiar with. And in which tests does it appear?
DAOS
It is a raw material for color development in the biochemical kit, CAS No.: 83777-30-4, is a white solid powder, has high water solubility, and is a stable aniline analog, which is used in the color development process and oxidation reaction. A wide pH range is suitable for diagnostic assays and biochemical tests. It has the advantages of good water solubility, high sensitivity and strong stability.
DAOS in blood glucose testing
The determination of glucose in blood is an important test item in clinical medicine. Novel Trinder's reagent DAOS coupled with 4-aminoantipyrine (AAP) as a reduced chromogen for the determination of glucose content.
DAOS in cholesterol detection
Through the coupling of DAOS and 4-AAP, a color reaction occurs with hydrogen peroxide under the catalysis of POD, and the concentration of total cholesterol can be determined by measuring the absorbance.
DAOS in triglyceride detection
When detecting triglycerides, triglycerides are hydrolyzed into glycerol and fatty acids in the presence of lipoprotein lipase (LPL); glycerol and ATP are catalyzed by glycerol kinase (GK) to generate glycerol-3-phosphate and ADP; glycerol-3- Phosphoric acid and oxygen are catalyzed by glycerol-3-phosphate oxidase (GPO) to generate dihydroxyacetone phosphate and hydrogen peroxide, and then the chromogenic substrate is coupled with 4-AAP and hydrogen peroxide to generate color as in the above steps. reaction, and the TG concentration was measured.
DAOS in high-density lipoprotein detection
When detecting high-density lipoprotein, it adopts a two-reagent reaction. Reagent 1 contains polyanion and surfactant, which selectively binds to VLDL and LDL, and inhibits COD and CEH in reagent 2. VLDH-CH, LDH-CH , so as to selectively act on HDL-CH, through the action of CEH-COD-POD, and then measure the absorbance to determine the concentration of HDL, and finally calculate the concentration of LDL.
Desheng is an old-fashioned manufacturer with more than ten years of R&D and production experience. Its main products include blood collection tube additives, biological buffers, new Trinder's reagents, chemiluminescence reagents, etc.