What are the anticoagulant mechanisms of edta-k2 and heparin sodium

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Anticoagulation mechanism of edta-k2

Edta-k2 is one of the most commonly used and important anticoagulants and reagents in clinical examinations. Its mechanism is to prevent blood coagulation by forming stable chelates with calcium ions in the aqueous phase. EDTA salts are potassium, sodium and lithium salts, all soluble in water, potassium salt is more soluble than sodium salt, the best use of EDTA potassium in whole blood cell count. EDTA can affect the activity of some enzymes and inhibit lupus erythematosus factor, so it is not suitable for the preparation of histochemical staining and examination of blood smear of lupus erythematosus cells. EDTA also affects platelet aggregation and leukocyte phagocytosis, and is not suitable for hemostatic tests and platelet function tests. Smear prepared with edta-k2 anticoagulant has an effect on granulocyte morphology, which is related to the time of anticoagulant placement and the concentration of anticoagulant.

Anticoagulant mechanism of heparin sodium

Heparin is the best anticoagulant in blood chemical composition determination. Heparin is a kind of mucopolysaccharides containing sulfuric acid group, molecular weight of 15000, its anticoagulant mechanism is with anticoagulant enzyme Ⅱ, at low concentrations can inhibit factor a and Ⅷ Ⅸ and PF3 between role, and can strengthen antithrombin Ⅲ inactivated serine protease, preventing thrombin formation; There is also the self-catalysis of inhibiting thrombin and the role of inhibiting factor X.


Heparin salts include sodium, lithium, and ammonium salts. The usual heparin anticoagulant dose is 10.0 ~ 12.5IU/mL of blood. Clinical commonly used heparin lithium, although its price is more expensive, but the anticoagulant effect is good. Because heparin sodium can increase plasma sodium content, and heparin ammonium can increase urea ammonia content. Some biochemical components differ significantly between serum and heparin anticoagulant plasma. The serum potassium content is higher than that of plasma due to the destruction of red blood cells during coagulation, so pay attention to the difference between serum and plasma when potassium ions are measured. In addition, excessive heparin can cause leukocyte aggregation and thrombocytopenia, so it is not suitable for leukocyte classification and platelet count, let alone hemostatic test.


The results show that heparin sodium anticoagulant has a significant effect on the determination of total protein content in blood, which is higher than that of 3% ~ 5% in serum. But after joining the anticoagulant, the total protein content is lower than the total serum protein content, the reason is that after joining sodium heparin anticoagulant blood, blocking the blood coagulation live enzymes generate, to prevent blood clots, to the hydrolysis of fibrinogen not into fibrin monomer, effectively prevent the formation of fibrin monomer, these still remains in the plasma fibrinogen. The serum, however, is the coagulation of blood, the hydrolysis of fibrinogen into fibrin monomer, and consumption due to coagulation, centrifugation with the blood cells were separated. Therefore, the total protein mass in plasma is all proteins including fibrinogen, while the total protein content in serum does not contain fibrinogen, which should theoretically be lower than the total protein content in plasma. Therefore in the determination of total protein content in blood, the best in serum specimens, for some emergency specimens, or some other reasons are in urgent need of determination of patients, need to do with the plasma samples determination of total protein, after the measured total protein should be deducted from the content of fibrinogen (usually deduct 3% ~ 5%), such ability and in serum samples of total protein content, reflect the total protein content of the patient's body.


As for the quality of EDTA dipotassium and heparin sodium produced by desheng technology, it has been completely able to compete with the imported enterprises. Our products have established long-term cooperative relations with many enterprises, and some customers have ordered 100 kg of EDTA dipotassium from desheng technology every month. We believe that good products and services will bring more long-term customers. To respect customers and achieve win-win results is the common goal of desheng and all major enterprises.