Desheng answers common questions about virus preservation solution in application
1. How to judge whether the virus transport media has deteriorated or grown bacteria?
Generally, it can be observed with the naked eye first, usually the non-inactivated virus transport media is easy to deteriorate or grow bacteria, and the inactivated type is not
Perishable. The deteriorating storage solution is usually uneven in color, accompanied by turbidity or flocculation, etc. Of course, the normal color will eventually be
It is necessary to determine whether it is available through testing. If you want to avoid spoilage and growth of bacteria in the preservation solution, you must keep a strict low temperature and keep the product container well sealed.
2. How can the virus transport media have different colors?
The active ingredients in the non-inactivated virus transport media are actually colorless. The reason for the color is that it contains
pH indicator phenol red. Phenol red is yellow when it is acidic, red when it is neutral, and purple-red when it is alkaline. Other types
The indicator is similar. By observing the color change, we can quickly determine whether the pH of the virus transport media has changed or
Bacteria growth and deterioration appear.
3. What is the nucleic acid direct expansion virus transport media
The traditional virus transport media requires nucleic acid extraction before amplification, while the nucleic acid direct expansion virus transport media can both inactivate the virus and directly amplify the sample after lysing! So that the entire virus nucleic acid detection time is greatly shortened! The virus nucleic acid can be stored at room temperature for 7 days without cold chain transportation. It is a safe and fast new crown detection tool under the current new crown detection form!
4. What should I do if I spill the virus transport media?
Answer: The virus transport media is basically the preservation solution before sampling and does not contain virus samples. Just need to rinse with water
That's it. If it is to operate the preservation solution of the collected virus samples, it is necessary to have a dedicated technician in a dedicated laboratory
For the operation, because the protective clothing, protective gloves, goggles and other equipment are worn in advance, the preservation liquid will not contact the skin.
5. How long can the virus transport media be stored?
At this specific time, different types of preservation solutions should be distinguished. Generally, before sampling, whether the virus transport media is inactivated,
Still non-inactivated type. It can be stored for months or even a year at room temperature. But the storage time after sampling is very limited
Limited. Because the characteristics of the virus and nucleic acid itself will affect the preservation time of the virus transport media, the preservation time does not depend on
It depends on the quality of the virus transport media.
6. The difference between virus transport media and cell preservation solution
Many people confuse the virus transport media with the cell preservation solution. In fact, the cell preservation solution is a general-purpose cell cryopreservation solution, which can be used to preserve animal and human cell lines. Cell preservation is a cytology experiment. Important technical means. In cell establishment and line establishment, it is very important to freeze the original cells in time. virus transport media is mainly used to preserve nucleic acids, while cell preservation solution is preserved by improving cell capacity and protection.
EDTA-2K is a commonly used anticoagulant, and its purple blood routine test tube specifications are diverse, suitable for blood collection and preservation. When using, attention should be paid to the collection method, anticoagulant ratio, storage temperature, avoiding shaking, and timely submission for inspection. In special circumstances, it is necessary to consider the patient's medical history and medication situation. Following these precautions can ensure the accuracy and safety of blood samples.