Multiple uses of cyclohexylamine propanesulfonic acid CAPS (1135-40-6)
Release time:
2022-01-08
3-cyclohexylamine-1-propanesulfonic acid is abbreviated as CAPS, and its CAS number is 1135-40-6. It is a commonly used Good's buffer in biological experiments. In addition, CAPS is a modified or new coating. An excellent modified raw material in the modification of materials, with a wide range of uses.
CAPS for biological buffer
When CAPS is used as a buffer, it has the same function as Tris base or phosphate buffer. It is used to adjust the pH value in the biological experiment system. This is also one of the buffers mainly produced by Desheng. Different from buffers such as Tris and Bicine, CAPS is suitable for weakly alkaline systems, and the buffer range is 9.7-11.1. In protein, DNA, RNA detection kits, CAPS is one of the commonly used buffers.
CAPS buffer powder
CAPS is used for protein electrophoresis experiments:
In fact, in many protein electrophoresis experiments, the running buffer used is Tris, such as Tris acetate TAE, as well as TBE, TPE, etc., but they are generally used for small nucleic acid or protein fragments, while Tris glycine buffer electrophoresis Later, it will have an impact on protein sequencing. The use of CAPS as the electrophoresis buffer is suitable for electrophoresis of high molecular weight proteins (greater than 20KD), that is, western blot. Since the electrophoresis buffer made by CAPS only contains EDTA and methanol, protein gel electrophoresis PVDF is not only used for protein separation, but also for subsequent protein sequencing.
CAPS for water-based coatings
CAPS is a zwitterionic sulfamate, which reacts with aliphatic polyisocyanate under mild conditions and in the presence of a tertiary amine neutralizer. The resulting sulfonic acid urea derivative is an excellent emulsifier, regardless of salt-forming groups. The CAPS-modified polyisocyanate has very good storage stability, and the finished product is not turbid. Even if it contains less sulfonate groups, it can also obtain a latex with a good dispersion state in water. Thus, a series of ionized modified polyisocyanates can be obtained, which can be used in various environmentally friendly high-quality water-based two-component polyurethane coatings. These coatings completely surpass general solvent-based coatings in terms of dryness, curing and chemical resistance. With the new regulations requiring further reduction of the VOC (Volatile Organic Compound) content in decoration materials, the amount of these cross-linking agents has been greatly increased. Compared with solvent-based coatings, they will not cause the quality of the paint film to decrease.
The above-mentioned introductions are several common uses of cyclohexylamine propanesulfonic acid CAPS, and their requirements for reagents are also different. For details, you can consult the manufacturer of Desheng, which can provide analytical pure and industrial-grade CAPS reagents.
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