Three common problems in the application of chromogenic substrate ADOS

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The chromogenic substrate ADOS is a kind of raw material for color development in the biochemical kit, CAS number: 82692-96-4, which can interact with 4-AAP .In the presence of peroxidase, it is catalyzed and oxidized by hydrogen peroxide to produce a color reaction. The color reaction is sensitive and the detection is rapid. The instructions for using the ADOS reagent produced by our company are provided here for reference only.

What should I do if the ice pack melts after ADOS receives the goods?

After receiving the goods, the built-in ice pack has melted, will it cause the product to deteriorate? This product is stable at room temperature. Low temperature transportation is to prevent extreme temperature conditions that may occur during transportation. Therefore, when you receive the product, if the ice pack has melted, it will not affect its quality, so please feel free to use it. If it is stored for a long time, it is recommended to store it in the cold and avoid light. If it is to be prepared into a solution, it is recommended to use it immediately to avoid oxidation and discoloration in contact with air.

What is the specific detection method of ADOS?

1. Prepare sample solutions for enzymatic oxidation reactions. The pH range of the buffer should be 5.5-9.5.

2. Use the same buffer to prepare a standard solution containing a known amount of substrate.

3. Add an appropriate unit of oxidase to the sample solution, and then add an equal volume of the detection solution.

4. Incubate the mixture at room temperature or 37°C for 30 minutes to 1 hour.

5. Prepare a standard curve and determine the concentration of the substrate in the sample solution.

ADOS reagent packaging and precautions for use:

Desheng's ADOS reagent packaging box is a foam insulation box, which contains an ice bag, and contains a product manual and test report. If it is not complete, please call customer service to request an electronic version. This product needs to be sealed and stored in a dry and cool place. It needs to be packaged in a dark brown bottle with better protection from light. ADOS is a white crystalline powder. If the color becomes darker, it may have grown bacteria or partially oxidized and cannot be used. ADOS powder may adhere to the wall of the tube or bottle when it is dissolved. It is allowed to use a centrifuge at low speed to reduce the loss of the product; the product has good solubility and is directly dissolved in deionized water; double steam can be used for higher experimental requirements Water or ultrapure water.

Desheng has 15 years of research experience in R&D and production of chromogenic substrates, and has mature technology and team. The purity of the ADOS products produced is greater than 99%. The product packaging is standardized to ensure safe transportation and timely after-sales service to ensure that customers can receive satisfaction. The product.