Common problems in the application of ethylenediaminetetraacetate tripotassium in blood collection tubes
Tags: Tripotassium, EDTA K3, Desheng
1. What is the coagulation mechanism of EDTA K3?
Answer: EDTA (ethylenediaminetetraacetic acid) and its salt are a kind of amino polycarboxylic acid (salt). EDTA salt chelates with calcium ions or other divalent ions in the blood sample, blocking these ions from playing the role of thrombin as a cofactor, blocking and terminating the endogenous or exogenous coagulation process, thereby preventing the blood specimen from clotting.
2. Does the EDTA tube need to be dried?
Answer: The ethylenediaminetetraacetic acid tripotassium tube does not need to be dried, but each manufacturer can still formulate its own production plan according to its own needs.
3. Tripotassium ethylenediaminetetraacetate is used as an additive for common blood routine tubes. What is the recommended dosage?
Answer: It is recommended to add 1.8mg per milliliter of blood as a blood anticoagulant. The reagent dosage can be seen in the following table.
EDTA K3 blood collection tube
4. What is the correct operation of the purple blood routine tube represented by EDTA K3 after blood sampling?
Answer: EDTA K3 should be inverted and mixed 5 to 8 times as soon as possible after blood collection to prevent the formation of small blood clots. Especially when the blood sampling temperature is higher than 25°C, the blood and EDTA K3 must be thoroughly mixed in time, otherwise it will easily cause blood coagulation or local coagulation.
5. Why do more than 100+ blood collection tube manufacturers in China choose Tripotassium produced by Desheng?
Answer: The tripotassium salt of ethylenediaminetetraacetic acid produced by Desheng is produced by itself and has independent intellectual property rights. The crystal shape is good, the purity is high, and the batch difference is stable. For many years, it has focused on serving more than 500 customers at home and abroad. It has rich clinical experience and is the best brand of choice for customers.
6. EDTA K2 and EDTA K3 are both additives of purple head cap blood collection tubes, so what is the difference between the two?
Answer: See the table below for specific differences.
Tripotassium ethylenediaminetetraacetate (EDTA K3) Lithium heparin
Coagulation mechanism Anticoagulant effect is achieved by reversible chelation with calcium ions in the blood. By activating antithrombin III to inactivate serine protease, it prevents the formation of thrombin and prevents platelet aggregation. effect
Action stage Endogenous or exogenous coagulation Multiple stages in vivo or in vitro
Scope of application Suitable for general hematology tests, not suitable for coagulation tests and platelet function tests, and not suitable for calcium ions, potassium ions, sodium ions, iron ions, alkaline phosphatase, creatine kinase and leucine aminopeptidase The determination is suitable for PCR test. It is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general energy biochemical determination, not suitable for blood coagulation test. Excessive heparin can cause the accumulation of white blood cells and cannot be used for white blood cell counting. Because it can stain the blood slice and the background is light blue, it is not suitable for white blood cell classification
7. Why do some EDTA K3 tubes show tiny coagulation fragments after blood sampling?
Answer: This is because the local anticoagulant concentration in the EDTA K3 tube is too high, causing part of the blood to enter the blood collection tube and fail to fully mix with EDTA K3, resulting in a part of micro clots. This may be caused by the failure to invert and mix the test tube in time after blood sampling.
8. In addition to being used as an additive for the purple headgear, can EDTA K3 also be used as an additive for other tubes?
Answer: Yes, it can be used in conjunction with sodium fluoride and separating glue. When EDTA K3 is used in combination with sodium fluoride, it can be used in blood glucose tubes, adding 1.2 to 2.0 mg per ml of blood. When EDTA K3 is used in combination with separating glue, the dosage of tripotass remains unchanged, and 0.8-1.2 grams of separating glue is enough for each tube.
9. What is the difference between tripotassium edetate as an anticoagulant and anticoagulant heparin salt?
10. What are the storage conditions of dipotassium ethylenediaminetetraacetate?
Answer: This product can be sealed and stored in a cool and dry place, protected from light and protected from moisture. The aqueous solution of this product can be sealed and stored at room temperature under a sterile state. It is recommended that the aqueous solution of this product be used immediately after preparation, and do not store in a solution state for a long time.
Tris, as the "leader" of the buffering agent family, ranks first in both customer registration and popular buffering agents in the market. Its raw material price and quality have become a focus of attention. As one of the suppliers of trimethylaminomethane, Desheng has been favored by a large number of customers due to its cheap raw materials.