Tris Acetate has a good reputation for many years--Desheng buffer
Many products of biological buffers are not just a name, but also have abbreviations and full names. CAS is also frequently searched. Here is an introduction to Tris Acetate, or TRIS Acetate for short, sometimes called "TAE". It is one of the more commonly used electrophoresis buffers and plays a large role in the production of agarose gels. Proportion.
It is very important to choose high-quality TRIS acetate, in order to achieve the purpose of producing better finished products. In our country, the use of TRIS acetate is not too common. Most of them are replaced by tris or other buffers. However, in order to produce good products more conveniently and quickly, many manufacturers choose good quality TRIS acetate. Salt.
Introduction of Tris(hydroxymethyl)aminomethane acetate:
Chinese name: Tris(hydroxymethyl)aminomethane acetate
Abbreviation: TRIS Acetate/TAE
Molecular formula: C4H11NO3.C2H4O2
Molecular weight: 181.19
Density: 1.09 g/mL at 20 °C
Boiling point: 219-220 °C (9.7513 mmHg)
Melting point: 120-121 °C
Storage conditions: sealed and protected from light at room temperature
Synthetic route: synthesis of tris(hydroxymethyl)aminomethane and acetic acid
Tris(hydroxymethylaminomethane) acetate application:
1. As a common buffer, it can be combined with glutamic acid to detect APT sensitively;
2. Used for non-denatured DNA electrophoresis buffer, supplemented when the electrophoresis buffer is insufficient for a long time;
3. Conduct various biochemical tests, scientific research experiments, etc.
Quality judgment of tris(hydroxymethyl)aminomethane acetate:
In addition to the price issue that buyers are very concerned about when choosing Tris Acetate, there is also a very key factor, that is the quality, purity and appearance of Tris Acetate, which can also directly reflect this product. The quality is good or bad.
In terms of buffers, purity is a more professional issue. Here is a brief introduction: under normal circumstances, buffers will have purity requirements. Industrial grades require 95% or 97%. The purity of analytical purity and some indicators will be required. Even higher, more than 99% are standard.
In terms of appearance, white crystalline powder is required, and the powder is required to be uniform. This is actually a requirement for the process. In fact, there are not many companies in China that can meet the purity and appearance.
Desheng is a blood reagent-based enterprise established in 2005. In 2011, it began the research and development and production of biological buffers. So far, it has successfully developed more than 20 buffer products and has formed mass production. Glycaminomethane acetate is produced and developed on the basis of tris, and the quality is also excellent. Business friends who need it can apply for sample testing first!
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.