The influence of different anticoagulants on the detection results of platelets and their parameters

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Platelet and its parameters are a dynamic index of blood examination, and its changes can provide accurate basis for the diagnosis and treatment of clinical bleeding diseases. As a routine item of clinical testing, its test results are easily affected by many factors. With the continuous development and wide application of automatic blood cell analyzers in recent years, how to choose a suitable anticoagulant is to test platelets and quality control of their parameters. One of the important factors. We select three commonly used anticoagulants, and use EDTA K2 as the standard to compare and analyze the effects of the other two anticoagulants on platelets and their parameters.



Strong anticoagulant, can inhibit the primary aggregation of platelets, make the platelets separated into single particles after in vitro, but have little effect on the platelet count, and have good stability. However, it has a certain influence on the determination of mean platelet volume (MPV) and has the effect of increasing its volume. The MPV of EDTA K2 anticoagulant blood is higher than the MPV value measured by the other two anticoagulants. It has been reported that it is due to the change of platelets from a disc shape to a spherical shape in the EDTA K2 anticoagulant. This difference should be noted in daily work.


Sodium Citrate

It is a calcium chelate, which is alkaline and can form a soluble chelate with calcium ions. Its anticoagulant property is weak, and it is easy to aggregate platelets and reduce the total number. The results of this group showed that compared with EDTA K2: anticoagulation, thrombocytopenia and PDW increased, the difference was extremely significant (P<0.01).


Lithium Heparin

The main function is to inhibit the formation of thromboplastin and thrombin, and activate plasmin. However, because heparin can cause platelet aggregation and swelling, or excessive heparin can cause platelet destruction, it will be treated as other cells due to its large size when counting. The results showed that the total number of platelets and PCT decreased significantly, while PDW increased. Compared with EDTA K2 anticoagulation, the difference was extremely significant (P<0.01).


It can be seen that how to choose a suitable anticoagulant is an important part of daily inspection work. The correct choice and use can accurately reflect the true condition of platelets in the blood and provide a strong basis for clinical evaluation of patient's platelet function. The recommended EDTA K2 anticoagulant is relatively stable for the determination of platelets and their parameters, with little impact, and is also stable for other routine hematology parameters. However, sodium citrate and lithium heparin have a greater impact on platelets and their parameters, and they are not suitable as anticoagulants for routine analysis of platelets and their parameters.


Whether it is a series of blood collection tube additives such as EDTA K2, sodium citrate or lithium heparin, our Desheng has more than ten years of R&D and production experience in this area, and all have obvious advantages. If you don't know how to choose, then come to our Desheng! We will lead you to understand the mystery.