Do you know the correct way to deal with blood anticoagulants?

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The laboratory makes hundreds of blood routines (purple hat), coagulation tests (blue hat), erythrocyte sedimentation rate (black hat), etc. every day. Once these specimens are clotted, their value is equivalent to medical waste. These coagulated anticoagulant specimens are more common in blood routines. Except for the technical aspects of blood drawing, it is often because the blood specimens coagulate due to incomplete mixing. Therefore, the correct method of specimen mixing is very important.


Anticoagulant specimens must be mixed upside down

Mix the blood immediately after drawing the blood. If you just shake it from side to side, it won’t work. Mix it upside down. Because the anticoagulant tube is transported over long distances, the anticoagulant may go to the lid very unobservantly, and there will be no or only a small amount of anticoagulant under the tube. If it is just swayed from side to side, it may not be fully anticoagulant, resulting in Blood clotting.


What is the correct mixing method of anticoagulant:

1. Green tube (anticoagulant: lithium heparin, sodium heparin), purple tube (anticoagulant: EDTA-K2 or K3), black tube (anticoagulant: sodium citrate: blood=1:4) after blood collection Immediately mix upside down 5-8 times.

2. Blue tube (anticoagulant: sodium citrate: blood = 1:9) immediately after blood sampling, invert and mix 3 to 4 times.

Although it's important to mix upside down, don't shake it vigorously. The blood cells are still very hypocritical. If the action is too violent-hemolysis, it will also affect the detection.


How long can the blood sample be stored with anticoagulant

Generally speaking, how long an anticoagulant tube can store blood is mainly determined by the anticoagulant substance in the anticoagulant tube, which mainly includes heparin and liquid. In addition, the storage time is different for different test items. If it is a morphological examination such as blood routine, it can be stored at 2 to 8 degrees for 7 days, if it is a biochemical test, it generally does not exceed 12 hours. If it is a blood glucose test, the result will be inaccurate after 2 hours without refrigerating and adding other reagents.


In addition, the inspector must use the anticoagulant correctly. Normally, clinical tests usually use serum as specimens and do not require anticoagulation. However, when anticoagulants are required for some special test items, attention should be paid to selecting the appropriate anticoagulant and the ratio of anticoagulant to blood. Prevent the specimen from clotting or changing the morphology of red blood cells.


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