Several biological buffers for cell culture

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One of the important characteristics of Good buffers (Hepes, Mops, Bicine, etc.) is that they are non-toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the pH of the experiment within a controllable range.

In the past few days, I want to learn more about the buffer system of cell culture, and I got some information about this by looking at professional literature. I will share and discuss it with you below, and hope that this information can help you choose buffers in the future. Time can make a difference.Several biological buffers for cell culture:

1. HEPES buffer

Effective buffer range: 6.8-8.2

pKa (25°C): 7.45-7.65

Ion binding: negligible metal ion binding

Used in cell culture: used to buffer mammalian cell culture in the open system of high-density culture and the closed system of high-density and low-density culture; used as a buffer for in vitro fertilization and embryo culture.


2. MOPS buffer

Effective buffer range: 6.5-7.9

pKa (25°C): 7.0-7.4

Ion binding: only interacts with iron

Used in cell culture: cell culture medium for yeast and mammalian cells. It should be noted that only concentrations below 20 mM are suitable for mammalian cell work; used in cell culture media to study the regulation of meiosis in mouse oocytes.


3. MES buffer

Effective buffer range: 5.5-6.7

pKa (25°C): 5.9-6.3

Ion binding: only interacts with iron

Used in cell culture: cell culture medium for yeast and mammalian cells. Please note that only concentrations below 20mM are suitable for working with mammalian cells; used in plant culture media (but the concentration is about 10mM-higher concentrations are toxic to most plants); used in culture media to promote pine and fir trees Growth.


4. MOPSO buffer

Effective buffer range: 6.2-7.6

pKa (25°C): 6.7-7.1

Ion binding: only interacts with iron

Used in cell culture: used as a buffer component of the charcoal yeast extract medium; used for the growth of the bacteria Legionella pneumophila.


5. TAPS buffer

Effective buffer range: 7.7-9.1

pKa (25°C): 8.25-8.65

Ion binding: interacts with Cu, Cr and Fe

Used in cell culture: Used for flagellate algae experiments in cell culture media.


6. BICINE buffer

Effective buffer range: 7.6-9.0

pKa (25°C): 8.1-8.5

Ion binding: interacts with Cu, Fe, Co, Mg, Ca, Ni, Zn and Cd

Used in cell culture: used for the cultivation of ammonia fungi.


The above are the recommended buffers for cell culture. Due to their special functions, many Good buffers are considered ideal buffers for cell separation, cell culture, enzyme analysis and many other biochemical applications.Nowadays, there are more and more applications of biological buffers, and the market demand is increasing, so many customers can't find the source of high-quality biological buffers. In this article, if you need a buffer, please contact our company. Hubei Xindesheng Material Co., Ltd. is a manufacturer specializing in the production of biological buffers. It will satisfy everyone in terms of price and product quality.