Problems of Serum Separating Gel in the Detection of Blood Collection Tubes in Patients with Multiple Myeloma
Nowadays, the blood collection tubes on the market are generally divided into blood anticoagulation tubes and blood coagulation promotion tubes. The ce items tested by different blood collection tubes are also completely different. Today, I’m talking about blood collection tubes that use serum separating gel. In the past, serum separating gel was mostly used in coagulant tubes and used in conjunction with blood coagulant. However, facing the higher requirements of the medical industry, The serum separation gel is not limited to the anticoagulant tube, and some are used with EDTA anticoagulant.
Of course, blood collection tubes with serum separation gel are more commonly used to detect serum. After centrifugation, serum and blood clots will be separated well. The good inertness of serum separation gel and the density different from serum and blood clots are used to form a Isolation layer. This also prevents the serum from being mixed with other cells and cannot be detected well.
In many cases, serum separation gel can separate serum and blood clots very well, but there are some exceptions in some cases. For example, in a 52-year-old man with multiple myeloma, the serum separation gel does not play a good role in separation. It is not the reason for the serum separation gel. On the contrary, even after the correct centrifugation, the serum separation gel is still Located in the upper part of the serum. It is the underlying disease of the patient that causes this result.
By checking the blood of a patient with multiple myeloma, his serum IgA concentration is 76.5g/L (reference range: 0.7-5.0g/L), and his total protein concentration is 140g/L (reference range: 66-83g/ L). This significant hyperproteinemia makes the serum density denser than the serum separation gel, so the gel is higher than the serum separation gel during centrifugation. This phenomenon can be seen in advanced myeloma cases; as an iatrogenic effect after administration of an iodinated radiocontrast agent; in dialysis patients, when blood is taken from the catheter system, which uses concentrated sodium citrate The solution serves as a blocking solution. This situation reminds us to consider multiple myeloma when we see this phenomenon.
MOPS buffer is an important biochemical reagent used to maintain the acid-base balance and ion environment of tissue samples, protecting cell structure and function. Widely used in cell culture, tissue fixation, and immunohistochemical staining to improve experimental accuracy and reliability. Understanding the characteristics and application principles of MOPS buffer is crucial for biomedical research.