Precautions for the use of anticoagulant dipotassium EDTA
Dipotassium ethylenediaminetetraacetate, dipotassium EDTA for short, is a very important anticoagulant in vacuum blood collection tubes. Compared with anticoagulants such as heparin, sodium citrate and potassium oxalate, it is used more frequently, so It is helpful to understand some of its usage details.
EDTA dipotassium anticoagulant powder
EDTA Dipotassium Application Type of Blood Collection Tube
The anticoagulant added to different types of anticoagulation tubes is naturally different. Dipotassium EDTA is usually added to the vacuum blood collection tube for blood routine testing, usually a tube with a purple safety cap. In addition, EDTA dipotassium is generally used as a blood anticoagulant in nucleic acid detection tubes. One is because EDTA can complex calcium ions in the blood and inhibit the clotting process; second, EDTA can also complex magnesium ions, so It has an inhibitory effect on nucleases that require the presence of magnesium ions to function, and can prevent nucleic acids from being degraded by nucleases.
EDTA dipotassium dosage
The amount of EDTA dipotassium in the blood collection tube is determined according to the amount of blood collected. According to the requirements of the YY0314-2007 standard appendix NC, the content of EDTA dipotassium or tripotassium in each ml of blood is 1.5-2.2mg, and the middle is taken when preparing reagents. Value 1.8mg/ml. It should be noted that the dosage of heparin anticoagulant is 20IU per milliliter of blood, one is measured by weight and the other is measured by titer. The two should not be confused.
EDTA dipotassium solution preparation
Dipotassium EDTA is a salt that is easily soluble in water. It can be directly dissolved in water when prepared. However, whether it is mixed with dipotassium EDTA or tripotassium anticoagulant with blood samples, there will be a small amount of dilution. In order to control the influence of dilution, the concentration of the prepared EDTA solution should not be too low. Usually, 20 microliters of dipotassium solution is added to the blood collection volume of 2mL or 3mL. When it is necessary to increase the amount of dipotassium, the method of increasing the concentration of dipotassium has priority over increasing the amount of spray. However, the concentration of dipotassium should not be too high, too high concentration may reduce the solubility of EDTA dipotassium crystals when the temperature is lowered, and excessive potassium ion concentration may cause the risk of hemolysis of blood cells.
The anticoagulation principle of EDTA dipotassium and EDTA tripotassium is the same. After being formulated into a solution, only the concentration of EDTA ion and potassium ion are different, and the pH is slightly different, and there are certain differences in specific test items. Desheng focuses on the development and production of in vitro diagnostic reagent raw materials, and can provide high-quality EDTA dipotassium, tripotassium or other anticoagulants.
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