The role of separation gel in progesterone detection
What is a separating glue?
Separation gel is a kind of polymer material. Because its density is between serum and (or) plasma and blood cells, it can form a gel barrier between blood clot and serum and (or) plasma after centrifugation, so it can quickly Separate serum and/or plasma from blood. In addition, the separation gel improves the stability of serum analysis, the sample can be transported and stored in the original tube, and can also prevent hemolysis during long-term storage. The separation gel has the risk of adsorption of lipophilic analytes and drugs, and the adsorption effect is related to the contact time and sample volume. In addition, some unstable analytes, such as therapeutic drugs and steroid hormones, are not suitable for long-term storage in the separation gel blood collection tube.
What is progesterone?
Progesterone is secreted by the corpus luteum and ovary. It is an intermediate metabolite of steroid hormone synthesis. Its physiological role is to make the endometrium that is already in the proliferative stage under the action of estrogen continue to develop and proliferate, thicken and enlarge, become soft and secrete mucus. Preparing for implantation of fertilized eggs plays an important role in maintaining normal menstrual cycle and normal pregnancy.
Why do progesterone testing?
The detection of progesterone can assist the clinical judgment of ovulation, corpus luteum function and pregnancy, and the accuracy of the results is very important. At present, many clinical laboratories use immunoassay systems to detect progesterone. The instructions clearly indicate that progesterone samples should not be stored in sampling bottles containing gel-type isolates to avoid delayed absorption, and each laboratory is required It is necessary to judge the suitability of the blood collection tubes and serum separation products used by them.
Comparison of two kinds of blood collection tubes for real-time detection of progesterone content
The immediate detection results of serum progesterone in ordinary blood collection tubes were higher than those in separate gel collection tubes. There was no significant difference in the detection results of progesterone between the two blood collection tubes in the low-value group; while the progesterone between the two blood collection tubes in the medium group and the high-value group There are obvious differences in the test results. The serum progesterone content in ordinary blood collection tubes without separation gel is generally higher than that in separation gel collection tubes.
The trend of the detection time of serum progesterone in ordinary serum blood collection tubes
The serum in the ordinary serum collection tube was stored at 2～8℃, and the progesterone test results did not change significantly within 72 hours. The test results showed that the serum was stored in the ordinary serum collection tube at 2～8℃ for 72 hours, the low value group, the median value There was no significant change in the progesterone content of the high-value group and the high-value group over time.
Change trend of serum progesterone detection time in separation gel blood collection tube
The serum in the gel-separated serum collection tube was stored at 2～8℃, and the progesterone test results did not change significantly within 72 hours. The test results showed that the serum was stored in the gel-containing serum collection tube for 72 hours at 2～8℃, low value group The content of progesterone in the middle value group and the high value group did not change significantly over time. However, for pregnant women and patients with high suspicion of high progesterone levels, the type of blood collection tube should be carefully selected, and the clinical reference range of progesterone values should be established for different types of blood collection tubes. For the same patient, the same tube must always be used during the test, and the two blood collection tubes cannot be mixed, so as to avoid large changes in the detection value of progesterone, which will affect the clinical diagnosis.
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.