Desheng teaches you three detection methods of luminol
Luminol believes that many people who like to watch reconnaissance films should know what it is. In many crime scenes, for blood stains that we can't observe with the naked eye, sprinkling with luminol reagent can show traces of blood stains, even after scrubbing. Or blood stains over a long period of time can still show its original shape, so luminol is widely used in criminal investigations. Speaking of this, many friends will start to be curious. Since luminol works so magically, how is it used? What is the detection method? Next, I will introduce three detection methods of luminol.
Luminol powder produced by Desheng
1. Catalyst rapid luminescence method
Because under normal circumstances, the chemiluminescence reaction between hydrogen peroxide and luminol luminescence system is very slow, but after adding some catalysts that will not affect the entire detection system, the reaction will become very rapid. Commonly used luminol catalysts include metal complexes: hemoglobin, peroxidase, and transition metal compounds: Fe3+, Fe2+, Mn2+, Cr2+, etc.
2. Indirect inhibitor determination method
In addition to the above-mentioned catalyst acceleration method, some organic substances can be used as inhibitors to inhibit the chemiluminescence of luminol. For example, reducing compounds containing phenolic hydroxyl groups can react with oxidants during the reaction to reduce the chemiluminescence of luminol. The concentration of oxidant is reduced, and the intensity of chemiluminescence is reduced, so as to indirectly measure this kind of organic substances.
3. Liquid chromatography (LC) separation and detection method
Luminol derivatives such as isoluminol (ABEI) are labeled on carboxylic acid and ammonia compounds, and after separation by high performance liquid chromatography (HPLC) or liquid chromatography (LC), they are then combined with Hydrogen peroxide-potassium ferricyanide reaction for chemiluminescence detection. Other separation methods can also be used, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, separating it by centrifugation and dialysis, and then performing chemiluminescence detection.
The above are the three commonly used luminol chemiluminescence methods. Desheng is a manufacturer specializing in the production of luminol. After years of research and development, the production technology has been quite mature and can produce high-quality luminol on a large scale. Please feel free to contact us if necessary!
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.