Several major applications of Tris acetate

Release time:


Tris acetate, like Tris hydrochloride, is an important derivative product of Tris. It has a wide range of applications in pharmaceutical and chemical industry, in vitro diagnostics, microbial culture, and new materials. Here are some examples of its main applications.


Prepare Tris-Acetate Buffer (Tris-Acetate Buffer)

Tris acetate can be directly dissolved in water to prepare Tris-Acetate buffer. Although it can be prepared directly with Tris and glacial acetic acid, the melting point of glacial acetic acid is only 16 degrees and it is volatile. It is not easy to transport and store when it is in a liquid state, and the amount is large. not very convenient.


Preparation of an electrolyte for lithium batteries

This electrolyte for lithium batteries suppresses the capacity decay of the battery at low temperatures during the charging and discharging process of the battery, and can reduce the amount of gas generated by the electrolyte under high temperature conditions, effectively preventing the battery from swelling, and improving the high temperature performance of the battery . The main raw materials of the electrolyte according to parts by weight are: 5-10 parts of dimethyl sulfoxide, 8-12 parts of hydroxyurea, 25-30 parts of cytarabine hydrochloride, 6-8 parts of polyvinylpyrrolidone, and bistrifluoromethyl 2-6 parts of potassium sulfonylimide, 1-3 parts of lithium hexafluorophosphate, 1-3 parts of 2,4-dichlorophenylhydrazine hydrochloride, 1-3 parts of tris(hydroxymethyl)aminomethane acetate, (4- Amidine phenyl) methanesulfonyl fluoride hydrochloride 1-3 parts, N,N-dimethyl-p-phenylenediamine oxalate 0.5-1.2 parts.


Tris acetate detects the mercaptan content in beer:

(1) Reduce the gold chloric acid solution with trisodium citrate solution to obtain the gold nanoparticle standard stock solution;

(2) To the gold nanoparticle standard stock solution, add mercaptoethanol solutions of different concentrations, and then add CuSO4 solution and tris-acetic acid buffer to obtain the mercaptoethanol standard working solution;

(3) Standard curve preparation: take the concentration of lg sulfhydryl standard working solution as abscissa, and the ratio of absorbance of sulfhydryl standard working solution series at 660nm and 520nm as ordinate, fit the standard curve formula;

(4) Detection of beer samples: After processing the beer, perform the detection as described in step (2) and substitute it into the standard curve formula to obtain the mercaptan content. The invention has high sensitivity, good selectivity, simple and convenient operation, low detection cost, and low requirements on laboratories.


The buffer prepared with Tris acetate can also be added with EDTA as the buffer in DNA nucleic acid electrophoresis, that is, TAE buffer, which can be prepared with Tris or Tris acetate. Desheng is a manufacturer of biological buffers and can provide various buffer raw materials such as Tris, MOPS, HEPES and their salts.