What is the difference between oxidase and dehydrogenase in enzyme preparations
Enzyme preparations are now widely used in diagnostics, medicine, food, industry and other fields, and there are many types of enzymes. Various enzyme preparations are used in biochemical testing. Among them, oxidase and dehydrogenase are two types of applications. Many and easy to confuse, here is the difference between the two types of enzymes.
Among the enzyme preparations are glucose oxidase and glucose dehydrogenase, acetyl CoA oxidase and acetyl CoA dehydrogenase, all of which can catalyze the oxidation of glucose or acetyl CoA. For most organic substances, dehydrogenation is actually equivalent to oxidation, so many people confuse or even equate the two. From the classification of enzymes, although both dehydrogenase and oxidase belong to oxidoreductase, they belong to different subclasses and catalyze different types of reactions.
Dehydrogenase and oxidase in enzyme preparation
Features of dehydrogenase:
The reaction catalyzed by dehydrogenase is not aerobic, and the electron acceptor is a reducing coenzyme, such as NAD, NADP or FAD. Common dehydrogenases such as acetyl-CoA dehydrogenase and glucose dehydrogenase in fatty acid oxidation. Dehydrogenase is often used to calculate the amount of ATP produced. Types of reactions catalyzed by typical dehydrogenases: alcohol hydroxyl groups are oxidized to produce aldehydes or ketones, such as malate dehydrogenase in the tricarboxylic acid cycle; aldehyde groups are oxidized to carboxyl groups, such as glycolytic glyceraldehyde phosphate dehydrogenase, etc.; α-keto acid decarboxylation, such as pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, etc.; saturated hydrocarbon chain dehydrogenation to generate double bonds, such as fatty acyl-CoA dehydrogenase.
The characteristics of oxidase:
The electron acceptor of oxidases is molecular oxygen, and the product is water or hydrogen peroxide, often requiring flavin prosthetic groups. Such as glucose oxidase (Glucose Oxidase), which is commonly used when measuring blood sugar. Xanthine oxidase used in purine metabolism also belongs to this category and is a target for the treatment of gout.
Dehydrogenase and oxidase cannot be mixed. For example, glucose dehydrogenase and glucose oxidase are different enzymes. The former catalyzes glucose to produce gluconolactone and uses NAD or NADP as electron acceptor; the latter produces gluconic acid and hydrogen peroxide. In addition to dehydrogenase and oxidase, oxidoreductase also has peroxidase (including peroxidase, catalase, and peroxidase) and oxygenase. Desheng is rooted in the field of in vitro diagnostics and can provide a variety of enzyme preparations for biochemical testing.
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