The development history of Tris, Hepes and other biological buffers
Release time:
2021-05-21
Biological buffers (Tris, Hepes, etc.) are a class of molecules that stabilize solutions and have existed in laboratory technology for many years. For example, natural buffer substances such as bicarbonate and carbonic acid are produced through the interaction of organisms and molecules, and play a role in maintaining pH balance.
New buffers are introduced every year, and these buffers are built on a foundation developed more than a century ago. This article will start to explore the basis of making biochemistry inseparable.
In the 1960s and 1980s, a project to determine the best buffers produced a list that is still vital in modern laboratories. "Good's Buffer" is produced or collected by Norman Good and his colleagues, and is selected according to many standards for research applications in the biological field. Some requirements are pKa between 6 and 8, high water solubility, stability and no exchange with membranes or biochemical reactions. The priority substances can also be prepared easily and safely.
Tris is one of the more valuable buffers in the laboratory and was recognized by Norman Good as early as the early 1960s. Tris was called THAM in therapeutics and soon assumed the role of science. The Tris and other reagents identified by Norman Good continue to act as a balancer in the buffer mixture by adjusting the pH to the prescribed range.
In 2007, two studies used new buffers to optimize data recovery for biochemical tests involving DNA. In an article on real-time polymerase chain reaction (PCR) analysis, researchers intend to use a combination of buffer and Tris to improve the quantification and yield of replicated DNA. By using HEPES, MOPS or TAE alone or together with Tris, the efficiency of reaction detection and measurement can be improved. Another project identified a pair of new buffers for plant DNA flow cytometry. These buffers are called "universal buffers" (GPB) and "woody plant buffers" (WPD), and can produce high-quality results from samples. GPB is more suitable for soft tissue plants, while WPD is better for tough tissues. These original materials have a higher productivity than the buffers previously used in this technology, indicating that it is possible to replace other solutions.
Today's researchers use decades of expertise to develop new and better compounds to stabilize biochemical reactions. The old buffer system can be applied to emerging technologies, but more often new solutions will be created to replace outdated solutions.
At present, there are not many domestic manufacturers of biological buffers, but with the development of science and technology, there are more and more applications of biological buffers, and there are already shortages in many places. Recommend a manufacturer here: Hubei New Desheng Material Co., Ltd.
As a manufacturer specializing in the production of biological buffers, Hubei New Desheng Materials Co., Ltd. provides a large number of high-quality Tris and many other types of biological buffers. Our company can customize the product specifications and after-sales service according to customer needs, and we can provide professional testing services and packaging customization services to special customers. In any case, Desheng company must be the only one for you to buy biological buffers and selected.
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