What is the saturation of Tris, Hepes and other biological buffers at 0℃
Release time:
2021-05-20
Biological buffers have different degrees of saturation. Saturation means that the solution of a certain substance cannot dissolve more of the substance. Some of these biological buffers have very low saturation points, while Hepes, for example, saturate at higher concentrations. In this article, our company will share the saturation concentration of some biological buffers at 0℃ and other data for everyone to discuss.
Buffer concentration (M) Buffer range
ACES 0.22 6.1-7.5
CAPS 0.47 9.7-11.1
MES 0.65 5.5-6.7
MOPSO 0.75 6.2-7.6
BICINE 1.10 7.6-9.0
CHES 1.14 8.6-10.0
HEPES 2.25 6.8-8.2
PIPES 2.30 6.1-7.5
TRIS 2.40 7.2-9.0
TES 2.60 6.8-8.2
MOPS 3.00 6.5-7.9
BES 3.20 6.4-7.8
HEPPS 4.58 7.3-8.7
Most biological buffers are known for their excellent water solubility. In fact, this was determined in 1960 when Dr. Norman Good’s team developed some compounds. However, the water solubility of each buffer is very different. I will also share some data on the solubility of biological buffers in water below.
Buffer buffer range Solubility
MES 5.5-6.7 10g/100ml
BIS-TRIS 5.8-7.2 20g/100ml
PIPES 6.1-7.5 not very soluble in water and becomes soluble when the pH is higher than 7.
MOPSO 6.2-7.6 10g/100ml
MOPS 6.5-7.9 10g/100ml
HEPES 6.8-8.2 70.36g/100ml
TRIS 7.2-9.0 40g/100ml
TRIS-HCL 7.2-9.0 8g/100ml
BICINE 7.6-9.0 16g/100ml
TAPS 7.7-9.1 5g/100ml
CAPSO 8.9-10.3 12g/100ml
CAPS 9.7-11.1 5g/100ml
The above are some data on biological buffers. I hope that these data can be helpful for your future work. Nowadays, biological buffers are in short supply in the market, making procurement very difficult. Here we recommend our company, Hubei New Desheng Materials Co., Ltd.
As a manufacturer specializing in the production of biological buffers, Hubei New Desheng Materials Co., Ltd. provides a large number of high-quality Tris and many other types of biological buffers. Our company can customize the product specifications and after-sales service according to customer needs, and we can provide professional testing services and packaging customization services to special customers. In any case, Desheng company must be the only one for you to buy biological buffers. select.
News
2023/09/27
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