What is the saturation of Tris, Hepes and other biological buffers at 0℃

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Biological buffers have different degrees of saturation. Saturation means that the solution of a certain substance cannot dissolve more of the substance. Some of these biological buffers have very low saturation points, while Hepes, for example, saturate at higher concentrations. In this article, our company will share the saturation concentration of some biological buffers at 0℃ and other data for everyone to discuss.


Buffer    concentration (M)      Buffer range

ACES            0.22                        6.1-7.5

CAPS            0.47                        9.7-11.1

MES              0.65                        5.5-6.7

MOPSO        0.75                        6.2-7.6

BICINE         1.10                         7.6-9.0

CHES           1.14                         8.6-10.0

HEPES         2.25                        6.8-8.2

PIPES          2.30                        6.1-7.5

TRIS            2.40                        7.2-9.0

TES             2.60                        6.8-8.2

MOPS         3.00                        6.5-7.9

BES            3.20                        6.4-7.8

HEPPS       4.58                        7.3-8.7


Most biological buffers are known for their excellent water solubility. In fact, this was determined in 1960 when Dr. Norman Good’s team developed some compounds. However, the water solubility of each buffer is very different. I will also share some data on the solubility of biological buffers in water below.


Buffer     buffer range     Solubility

MES        5.5-6.7       10g/100ml

BIS-TRIS     5.8-7.2      20g/100ml

PIPES       6.1-7.5       not very soluble in water and becomes soluble when the pH is higher than 7.

MOPSO       6.2-7.6      10g/100ml

MOPS         6.5-7.9      10g/100ml

HEPES        6.8-8.2       70.36g/100ml

TRIS          7.2-9.0       40g/100ml

TRIS-HCL      7.2-9.0       8g/100ml

BICINE        7.6-9.0        16g/100ml

TAPS    7.7-9.1      5g/100ml

CAPSO   8.9-10.3     12g/100ml

CAPS     9.7-11.1     5g/100ml

The above are some data on biological buffers. I hope that these data can be helpful for your future work. Nowadays, biological buffers are in short supply in the market, making procurement very difficult. Here we recommend our company, Hubei New Desheng Materials Co., Ltd.


As a manufacturer specializing in the production of biological buffers, Hubei New Desheng Materials Co., Ltd. provides a large number of high-quality Tris and many other types of biological buffers. Our company can customize the product specifications and after-sales service according to customer needs, and we can provide professional testing services and packaging customization services to special customers. In any case, Desheng company must be the only one for you to buy biological buffers. select.