Brief Introduction of Dry Chemiluminescence Immunochromatography Test Paper
We all know that commonly used chemiluminescence reagents such as acridinium esters, luminol, ALP substrates, etc., all perform chemiluminescence detection in a solution environment, that is, wet chemical methods. In fact, they can also be used in dry chemical methods, such as Dry chemiluminescence immunochromatography test paper.
Chemiluminescence reagents such as acridinium esters have only been gradually replaced by domestic products in recent years. The corresponding CLIA technology of chemiluminescence immunoassay is in the development stage, so the common immunochromatographic test papers on the market are mainly colloidal gold, latex or fluorescence. . However, their sensitivity and anti-interference are not superior to chemiluminescence methods.
Dry chemiluminescence immunochromatography test paper and liquid adding tube
The current common chemiluminescence CLIA is mainly a wet chemical method that reacts with a solution in an enzyme-labeled plate or test tube. It has the advantage of high sensitivity and requires relatively high requirements for chemiluminescence equipment, and it is not suitable for single-person operation; on the other hand, , Colloidal gold or fluorescent-labeled chromatography test paper needs to be given a certain light source for detection. Colloidal gold is visible light, and fluorescence is excitation light. These lights are emitted by LED lights, which are prone to differences between lamps and lamps, which can lead to Differences in test results.
Based on the above two reasons, dry chemiluminescence immunochromatographic test papers have been gradually developed. This test paper and its detection method have many advantages. It emits light after the reaction without additional light source, which basically eliminates background interference, combines the convenience of immunochromatographic test paper, and also has the high sensitivity of chemiluminescence. The advantage of improving the sensitivity of the diagnostic kit.
In chemiluminescence immunoassays, whether it is dry or wet, acridinium esters can be directly labeled with antibodies or nucleic acids, but usually cannot be directly labeled with luminol. Instead, use HRP labeling, and the sensitivity of acridinium esters is higher. Of course, the reagents The cost is higher. Desheng is a manufacturer that develops and produces acridine esters, and also produces luminol and isoluminol. You can choose reagents according to actual testing requirements.
Choosing high-quality BES buffer is the key to ensuring experimental accuracy and reproducibility. Purchasing genuine BES buffer can ensure quality, fast supply, and professional service. When selecting genuine products, it is important to understand the product source, inspect packaging and quality, verify supplier qualifications, and refer to peer opinions to ensure experimental quality and efficiency.