Desheng answers common questions about virus transport media in application
1. How to judge whether the virus transport media has deteriorated or grown bacteria?
Generally, it can be observed with the naked eye first. Usually, the activated virus storage solution is easy to deteriorate or grow bacteria, and the inactivated type is not easy to deteriorate. Deteriorated storage solutions are usually not uniform in color, accompanied by turbidity or flocculents, etc. Of course, the normal color is ultimately tested to determine whether it is usable. If you want to avoid spoilage and growth of bacteria in the preservation solution, you must pay attention to maintaining a strict low temperature and the product container is well sealed.
2. How can the virus transport media have different colors?
The active ingredients in the activated virus transport media are actually colorless, and the reason for the color is because the pH indicator phenol red is added to it. Phenol red shows yellow when it is acidic, red when it is neutral, and purple-red when it is alkaline. Other types of indicators are similar. By observing the color change, we can quickly determine whether the pH value of the virus transport media has changed or whether bacteria have grown or deteriorated.
3. What is a nucleic acid direct expansion virus transport media
The traditional virus transport media requires nucleic acid extraction before amplification, while the nucleic acid direct expansion virus transport media can both inactivate the virus and directly amplify the sample after lysing! So that the entire virus nucleic acid detection time is greatly shortened! Viral nucleic acid can be stored at room temperature for 7 days without cold chain transportation. It is a safe and fast new crown detection tool under the current new crown detection form!
4. What should I do if I spill the virus transport media?
Answer: The virus transport media is basically the preservation solution before sampling and does not contain virus samples. Just rinse with clean water. If it is to operate the virus transport media of the collected virus samples, it needs to be operated by specialized technicians in a special laboratory. Because the protective clothing, protective gloves, goggles and other equipment are worn in advance, the virus transport media will not contact the skin. of.
5. How long can the virus transport media be stored?
This specific time also needs to distinguish between different types of virus transport media. Generally, before sampling, the virus transport media is either inactivated or activated. It can be stored for months or even a year at room temperature. But the storage time after sampling is very limited. Because the characteristics of the virus and nucleic acid itself will affect the preservation time of the virus transport media, the preservation time does not depend on the quality of the virus transport media.
6. The difference between virus transport media and cell virus transport media
Many people confuse the virus preservation solution with the cell preservation solution. In fact, the cellvirus transport media is a universal cell cryopreservation solution, which can be used to preserve animal and human cell lines. Cell preservation is one of the cytological experiments. Important technical means. In cell line establishment and line establishment, it is very important to freeze the original cells in time. . virus transport media is mainly used to preserve nucleic acids, while virus transport media is preserved by improving cell capacity and protection.
In chemiluminescence analysis, the luminescence intensity of acridine ester is influenced by various factors, such as reaction medium, temperature, time, and excitation light source energy. To achieve good detection results, it is necessary to comprehensively consider and optimize these factors. Meanwhile, attention should be paid to controlling and standardizing experimental conditions to ensure accurate and reliable results. Thoroughly studying these influencing factors will help promote the development of chemiluminescence analysis methods.