Desheng takes you to understand the direct-expanded virus transport media in one article
In order to match the downstream POCT instrument to obtain the experimental results quickly and conveniently, there are also high requirements for the virus transport media: firstly, it can inactivate the virus to ensure the safety of contacts; secondly, there are no components in the preservation solution that inhibit downstream reactions to ensure the experiment Go smoothly; finally, nucleic acid extraction can be eliminated, the overall detection time can be minimized, and the efficiency of nucleic acid detection can be improved. However, not all virus sample preservation solutions are suitable for downstream POCT diagnostic equipment.
At present, the newly launched "Cai Li Xing" inactivated viral nucleic acid direct expansion virus transport media can meet various conditions at the same time, perfectly match the downstream POCT diagnostic instrument, and is suitable for the one-step rapid virus nucleic acid detection system on the market. The following nucleic acid direct-expanded virus transport media are collectively referred to as "Cai Li Xian". Today, Desheng will take you to understand the direct-expanded nucleic acid virus preservation solution in one article.
Features of DNA Direct-Expanded virus transport media
1. Inactivate the virus: It can inactivate the virus without heat inactivating the sample to ensure the safety of contacts;
2. Nucleic acid direct amplification: after sample collection, nucleic acid extraction is not required and can be directly used as a template for amplification (for some downstream POCT instruments, it is necessary to add lysis solution to the sample preservation solution for amplification);
3. Non-guanidine salt components: the preservation solution does not contain guanidine salt, which does not inhibit downstream experiments such as qPCR and isothermal amplification;
4. Storage at room temperature: Viral nucleic acid can be stored at room temperature for 7 days without cold chain transportation.
Study on the effect of "Cai Li Xian" on the inactivation of poultry coronavirus
Under strict laboratory management practices, the SPF chicken embryo infection experiment is used to explore the effect of "Cai Li Xian" on IBV virus inactivation, observe the IBV nucleic acid detection amplification curve in the allantoic fluid, and obtain some experimental results, fluorescence In the quantitative PCR experiment, it was found that the "Cai Li Jian" was very thorough in killing the virus, and the nucleic acid of Avian Infectious Bronchitis Virus was almost undetectable. Therefore, the "Cai Li Jian" virus transport media can inactivate the virus very well.
Comparison of RNA Protection Ability of "Cai Li Jian"
"Cai Li Xing" can store RNA at 37°C without degradation, avoiding the degradation of nucleic acid in the sample before testing, resulting in "false negative" test results. We compared the effects of storage solutions containing guanidine salts on the market and "Cai Li Xian" stored at 37°C for IBV for 1-7 days. The results showed that there was basically no degradation of the viral nucleic acid in the "Cai Li", while the degradation rate of the viral nucleic acid in the other two virus transport media gradually increased, and it was almost completely degraded by the 7th day.
"Cai Li Jian" analysis of whether downstream amplification is inhibited
Most storage solutions on the market will significantly inhibit amplification when matched with POCT detection equipment, causing amplification failure. Dilute the IBV stepwise (10-3, 10-4, 10-5, 10-6) with "Cai Li Xian", and perform fluorescence quantitative PCR detection on the two sets of experimental samples at the same time. Experimental results: The sensitivity of the two groups is the same.
Therefore, the "Cai Li Xing" inactivated virus nucleic acid direct expansion virus transport media can not only inactivate the virus but also directly amplify the sample after lysing! So that the entire virus nucleic acid detection time is greatly shortened! It is a safe and fast new crown detection tool under the current new crown detection mode!
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