Protein PVDF Transfer Membrane Buffer CAPS
Release time:
2019-08-12
CAPS is a widely used buffer, also known as N-cyclohexyl-3-aminopropane sulfonic acid. Many biological tests and biochemical kits are used.
Now the PVDF transmembrane of protein has not used Tris-gly, SDS, 20% methanol buffer, because there is glycine in the buffer, so it is difficult to distinguish whether glycine is brought in by Buffer or the protein itself when sequencing. Now the transmembrane buffer used for sequencing is 10 mM/1 CAPS 10% methanol, and the methanol concentration standard in CAPS imprint buffer. In general, the buffer with high methanol
concentration has a good effect on the transfer of low molecular weight proteins, while the buffer with low or no methanol concentration is beneficial to the transfer of high molecular weight proteins.
When rotating the membrane, we should prepare the electroplating groove, electrophoresis apparatus, 0.45um (suitable for 20KD above protein) and 0.22um (suitable for 20KD below protein) pore size PVDF film, cut slightly larger than the gel size, two Whatman Whatman 3MM 3MM filter paper, cut into gel size, methanol (100%), DD water. CAPS imprinting buffer (mother liquor) is dissolved in deionized water, and the pH value is adjusted to 11.0 with 2mol/L_NaOH_. Then it is stored in 4_C at constant volume. CAPS imprinting buffer (working solution, 1 x CAPS containing 10% methanol, mixed before use) dyeing solution 0.1% Coomassie brilliant blue G-250_40% methanol/1% acetic acid, decolorizing solution 50% methanol.
DeSheng Science and Technology has been developing and producing reagents for in vitro diagnosis of blood testing since 2005. It has made a deep research on new Trinder's reagents, biological buffers and chemiluminescent reagents for chromogenic substrates.
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