The reason why the blood collection tube of the coagulant causes the needle to block the alarm of the biochemical instrument

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Some manufacturers who do biochemical testing of vacuum blood collection tubes reacted that the automatic biochemical analyzer tester sampling link on the vacuum blood collection tube coagulant tube with serum separation gel added, and occasionally the probe of the biochemical analyzer to absorb the serum sample would be blocked. The situation where the needle triggers an instrument failure alarm.


Then, some people will naturally think that the cause of the serum separation gel caused the error alarm of the biochemical analyzer. After all, when the coagulant tube with no separation gel is added to the machine for aspiration detection, there will be no alarm for the aspiration probe to block the needle. Situation, is it really the probe plugging caused by the separation glue?

The cause of the alarm of the biochemical instrument caused by the coagulant blood collection tube

Many domestic companies have not solved this problem. Desheng has also tested it repeatedly and finally confirmed that it is not the problem of the separation glue that caused the probe to block the needle. Studies have found that the root cause of the biochemical instrument blocking needle alarm is due to the non-standardized use of coagulation blood collection tubes. It is the fibrin filaments that cause the needle blocking, rather than the separation glue, which causes the needle to block.


Generally, there are the following reasons for the precipitation of fibrin: Use coagulation blood collection tube or separating gel to promote coagulation blood collection tube. To make the coagulant evenly distributed in the blood, it must be slightly inverted and mixed 4 to 5 times to make the center and surroundings of the blood collection tube The blood clots at the same time. If the blood is not completely coagulated and then centrifuged, fibrin can be precipitated. The upper part of the serum appears jelly-like and fragmented, which is mixed with the blood. Fibrin filaments are caused by the fact that fibrin has not fully contracted. Secondly, when the blood collection tube company prepared the coagulant blood collection tube, the coagulant spray was insufficient or the prepared water-soluble coagulant was not used up on the same day, and continued use the next day, resulting in the decrease of the coagulant effectiveness. The fibrinogen in the blood is gradually transformed into insoluble fibrin under the action of the coagulant. If the coagulation dose is insufficient or ineffective and the coagulation time is prolonged, centrifugation can lead to the precipitation of fibrin.


The reason why the coagulation tube without separation gel will not block the needle is that even if the blood of the sample in the tube is not completely coagulated, the fibrin filaments produced will sink to the bottom of the serum without the separation gel and will not be absorbed by the probe. , There will be no blocking of the needle.