Application characteristics of 3-cyclohexylamine propanesulfonic acid CAPS
Release time:
2021-02-18
3-cyclohexylamine propanesulfonic acid CAPS (CAS1135-40-6) has a molecular weight of 221.31. It is usually used as a biological buffer in related research experiments on several macromolecular substances such as nucleic acid and protein. It is similar to Tris, MOPS, Bicine, etc. but also has The difference is different, so what are its characteristics in application?
Cyclohexylamine propane sulfonic acid has a pKa of 10.4 under standard conditions (standard atmospheric pressure 25 degrees Celsius). The appearance is white powder and fine crystals. The density is slightly greater than water. It is soluble in water but not very soluble. 100g of water can dissolve 9g of CAPS at room temperature, and its solubility in hot water will increase significantly. Therefore, in production, CAPS is purified by dissolving CAPS in hot water, and then adding ethanol for cooling and crystallization.
Features of cyclohexylamine propanesulfonic acid in biochemical applications
From the dissociation constant value of cyclohexylamine propanesulfonic acid, we can know that CAPS is weakly basic. Although the propanesulfonic acid group in the molecule is weakly acidic, the cyclohexylamine group is basic to make the whole molecule appear Weakly alkaline. Therefore, CAPS buffers are usually used in biological experiments with a pH in the range of 9.4-11.4 (alkalescence).
And buffers such as Tris, Bicine, TAPS, the applicable pH range is usually close to the neutral pH 7, so CAPS is used as a buffer when separating basic drugs by HPLC. Moreover, CAPS is also suitable for the reaction system of enzymes with higher pH values, such as supporting the activity of alkaline phosphatase.
Cyclohexylamine propane sulfonic acid CAPS can reduce the yield of non-specific products of nucleic acid hybridization. When nucleic acid extraction, CAPS and cholicamidopropyl dimethyl ammonium propane sulfonate (CHAPS), cyclohexylamine hydroxy propane sulfonic acid are usually used. CAPSO and CHES are used together to prepare nucleic acid hybridization buffers, which can improve the specificity of nucleic acid hybridization, maintain the yield of target hybridization products, and play an important role in specific pathogen detection and gene sequence analysis.
CAPS is suitable for the separation and purification experiments of high molecular weight proteins (molecular weight greater than 20KD). In the protein PVDF transfer experiment, using CAPS instead of the Tris-glycine+20% methanol buffer system can reduce the amount of methanol, and it can also eliminate the interference caused by the glycine brought in the buffer during subsequent protein sequencing.
The above are the application characteristics of cyclohexylamine propane sulfonic acid CAPS, which are also based on the feedback of Desheng customers. There are also some CAPS application characteristics in DNA and RNA-related experiments and the application of biochemical kits. We need everyone Summarize in practice.
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