Heparin sodium is a kind of natural anticoagulant substance of acidic mucopolysaccharide containing sulfate group; it is mainly used for blood anticoagulation in blood collection tube additives, and achieves the function of preventing blood from clotting by inhibiting the activity of thrombin. Those who know it know that heparin sodium is purchased and used according to its potency. Therefore, we all need to understand the factors that affect its potency, and the high value of heparin itself is also to avoid unnecessary losses.

1. The effect of dissolution temperature on dissolution time and heparin sodium titer

The titer of heparin sodium showed a trend of increasing first and then decreasing with the continuous increase of temperature. The titer of heparin sodium reached the highest value at 40℃. If the temperature is too low, the crude heparin will take a long time to dissolve, resulting in waste of production costs; if the temperature is too high, the activity of heparin sodium will be destroyed and the titer of heparin sodium will decrease sharply. Considering the potency of heparin sodium and the cost of dissolution time, the optimal dissolution temperature is 40℃.

2. The effect of salt concentration on the titer of heparin sodium

Proper concentration of salt solution can separate protein from heparin, and when the salt concentration is too low, the impurity protein and heparin are not separated completely, which affects the activity of heparin. When the salt concentration is too high, the high concentration of salt solution will destroy the structure of heparin. , Resulting in reduced heparin activity. When the concentration of sodium chloride reaches 0.35mol/L, the titer of heparin sodium reaches the highest value.

3. The effect of heat denaturation time on the titer of heparin sodium

With the extension of heat denaturation time, the titer of heparin sodium increases first and then decreases. This may be due to the fact that the impurity protein gradually denatures over time during the heat denaturation process. When the time is too long, the structure of heparin under high temperature Damaged, thereby affecting the activity of heparin, resulting in a gradual decrease in potency. The best thermal denaturation time in the thermal denaturation process is 10min.

4. The effect of hydrogen peroxide dosage on the titer of heparin sodium

Excessive amount of hydrogen peroxide leads to the destruction of the molecular structure of heparin sodium, which makes heparin sodium partially inactivated, causing the potency to increase first and then decrease. Therefore, the optimal amount of hydrogen peroxide is when the ratio of the volume of hydrogen peroxide to the volume of the solution is 1%.

As a manufacturer of heparin sodium, Desheng would like to remind you:

1. Do not use the crude product directly, but use the refined heparin sodium, which is powdery and white in appearance.

2. After heparin sodium is dissolved in water to prepare a solvent, it can be sprayed directly on the wall of the blood collection tube to have an anticoagulant effect.

3. The conventional dosage is 1mL of blood corresponding to 20IU of heparin sodium. Excessive amount will not achieve the best anticoagulant effect.

4. Preservation method configured as a solution: 0-4 degrees low temperature storage, because heparin sodium cannot be stored for a long time after preparation, it should be used freshly, and the maximum storage period should not exceed 7 days.