What is the difference between the new Trinder's reagent TOOS, ALPS, MAOS, etc.
In enzyme-linked immunoassay, horseradish peroxidase HRP has a variety of substrates. Among them, the new Trinder's reagent is a kind of chromogenic substrate with high sensitivity and low interference. Such reagents include more than ten kinds of TOOS, ALPS, MAOS, etc. Types, what is the difference between them?
The new Trinder's reagent is developed and improved on the basis of the traditional chromogen substrate phenol or aniline. On the basis of the traditional chromogen, some functional groups are substituted and modified to make the chromogen water-soluble and reagent compatible. , Stability, changes in the maximum absorption wavelength of the color-developing product, etc., Desheng Technology has focused on the development of ten new Trinder's reagents for chromogen substrates.
The difference between the new Trinder's reagent TOOS, ALPS and MAOS
We know that the detection principle of Trinder's reagent is to use it to participate in the reaction to generate a color product with a specific absorption wavelength, and then use spectrophotometry to measure the absorbance change at a specific maximum absorption wavelength to analyze the concentration of the analyte . Then the maximum absorption wavelength and light absorption intensity of the color reaction products of different chromogen substrates determine the performance difference of chromogen substrates.
Through the Trinder reaction test, we found that:
The experimental results show that the chromogen substrates ALPS, TOOS, TODB, TOPS have high molar absorbance, which means that the sensitivity of the color reaction is high, and it can be used for the determination of very small amounts of test items in the serum. The absorption wavelength of chromogen MAOS and MADB is higher, reaching 630nm, which is less interfered by other substances in serum or other samples, and can be used for detection items that require high precision values.
In addition, in terms of the fading of the color product, the absorbance of the color reaction product formed by TOOS, ADOS, and DAOS does not change much with time, that is, the color reaction product is stable and not easy to fade. However, the product of MAOS fades significantly, and the data needs to be observed in time when testing.
The differences between different Trinder’s reagents can be summarized as follows:
1. TOOS has good performance in absorption wavelength, color reaction intensity and color fading, so TOOS is the most commonly used.
2. Of course, for a specific item to be tested, its content is extremely small, so ALPS and TODB are needed.
3. If some items to be tested are in 550nm~600nm, there will be interference, then you can choose MADB, MAOS.
In practical applications, the new Trinder’s reagents corresponding to some indicators are not very different and can be used interchangeably. Therefore, you can see that different companies’ diagnostic materials or kits use different chromogen substrates when testing the same biochemical indicator. Ten kinds of chromogen substrates developed by Desheng can meet the needs of different companies.
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