Bicine is used as a running buffer
Release time:
2021-01-16
Dihydroxyethylglycine Bicine buffer is a very versatile pH buffer. It is usually used as a buffer in the determination of the activity or content of various enzymes. In addition, it is also used as an electrophoresis buffer. It is a nucleic acid and protein coagulation buffer. An important component of the gel electrophoresis buffer system is the conductor in the electrophoresis field, which is also a necessary condition for maintaining a constant pH value of the electrophoresis system.
Dihydroxyethylglycine Bicine running buffer
Electrophoresis (Electrophoresis, EP) refers to the experiment in which charged particles move towards the opposite electrode under the action of an electric field. The composition of the electrophoresis buffer and its ionic strength affect the electrophoretic mobility of the particles. The buffer also needs to avoid chemical reactions with the separated sample to change the physical and chemical properties of the sample or make it lose its biological activity. EDTA is usually added to the electrophoresis buffer, which can chelate divalent cations such as Ca and Mg ions, which can prevent the activation of DNase and the precipitation of Mg ions and nucleic acids during electrophoresis.
The electrophoresis buffer refers to the electrophoresis medium used in the electrophoresis of nucleic acid DNA, RNA or protein molecules, to complete the electrophoresis experiment while stabilizing the pH of the system. During electrophoresis, both the anode and the cathode will undergo an electrolytic reaction, so the anode will become acidic and the cathode will become alkaline during a long period of time. The change of pH has a relatively large impact on protein or nucleic acid, and hydroxyethyl glycine Bicine buffer has a strong buffering capacity, so that the pH of the two poles of the solution remains basically unchanged in the case of long-term electrophoresis.
Another function of the electrophoresis buffer is to make the solution have certain conductivity to facilitate the migration of DNA, RNA or protein molecules. For example, the general electrophoresis buffer should contain Na+ ions that increase conductivity, and the concentration should be controlled at 0.01-0.04mol/L. When the concentration of Na+ ions is too low, the electrophoresis speed will be significantly slower; if it is too high, it will cause excessive current to heat the gel or even melt, causing the experiment to fail.
In some nucleic acid and protein electrophoresis buffers, there is also a component of EDTA, added at a concentration of 1-2mmol/L, the purpose is to chelate Ca, Mg2+ plasma and prevent the activation of DNase during electrophoresis.
In addition to Bicine, common running buffers include Tris, MOPS and CAPS. Sometimes different proteins or enzymes need different buffers for electrophoresis. In response to this, Desheng produces a variety of raw materials for electrophoresis buffers.
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