Introduction to the characteristics and advantages of the chromogen substrate MAOS
MAOS (CASNo.: 82692-97-5) full name: N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate, it is a new Trinder's reagent A very important member of the family. It is not as commonly used as TOOS, but it also has an absolutely irreplaceable effect. Like other new color reagents, it has high water solubility and sensitivity much higher than the traditional color reagents commonly used in some laboratories, so it is widely used in clinical testing and biochemical kits.
MAOS has a high absorption wavelength
The biggest feature of MAOS is that the maximum absorption wavelength of the oxidized product is much higher than that of ordinary color reagents. Even in the new Trinder’s reagent, the UV absorption wavelength of its product is quite high at 630nm. The maximum absorption wavelength of many color reagent products is in the visible light region. If it is to detect human blood or other body fluids, because the components in the sample to be tested are more complicated, the sample itself contains a small amount of components that absorb wavelengths in the visible light region or absorb with the color product The wavelengths are similar, which makes the detection result higher. If you use MAOS, the maximum absorption wavelength of the product is in the ultraviolet region and is relatively high, which will greatly reduce the interference. Therefore, for some biochemical testing items that require highly accurate values, it is recommended to use MAOS as a chromogenic substrate.
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PH value of MAOS
Secondly, the color reaction of MAOS chromogenic substrate has a wide range of adaptation to the pH of the reaction system, which greatly improves its adaptability. The reaction of some color reagents is often under acidic conditions, but the biochemical detection usually requires the participation of enzymes. We know that the catalytic action of enzymes is more sensitive to the pH of the reaction environment, and does not match the color reagents. Inactivation will be greatly restricted in use, but MAOS reagent does not have this problem.
Molar absorption intensity of MAOS
Another point is that the molar absorption intensity of the MAOS color reaction is relatively high, so its sensitivity is relatively high. It should be noted that MAOS will fade when the detection time is too long, so MAOS detection must be completed in time and cannot be interrupted. In addition, MAOS is also relatively safe, unlike DAB and other biphenyl color reagents, although the price is relatively low, but
There is a certain degree of carcinogenicity or mutagenicity.
MAOS is sensitive to light and humidity, so it has high storage requirements. It needs to use shading and well-closed containers. The temperature should be kept at 0-5℃, preferably refrigerated storage. At the same time, it should be protected from light and moisture to ensure detection. The quality is more accurate and higher.
Desheng Technology has been committed to the development, production and sales of chromogenic substrates, especially TOOS, MAOS, ADPS, etc., which have a good reputation at home and abroad, and have a good cooperative relationship with many biochemical kit manufacturers. Let us Together to contribute to the field of domestic diagnostic reagents!
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