What are the advantages of the preservation solution reagent for inactivating the virus
Release time:
2020-12-16
The preservation solution reagent for inactivated virus usually refers to the preservation solution of inactivated virus samples. Unlike the activated preservation solution, it does not need to retain virus activity and can quickly kill the virus without detecting subsequent viral nucleic acid. Make an impact.
Because the inactivated Virus Transport Media is highly efficient in inactivating the virus, it is safer than the activated preservation solution in the sampling and detection process, and will not cause secondary infection, so the operating requirements and experimental environment requirements are slightly lower . It is more advantageous for the efficiency of rapid nucleic acid detection and the calculation of detection coverage.
Preservative reagent for inactivating virus in sampling tube
This type of virus sample preservation solution can quickly inactivate the virus because it contains a high concentration of lysis salt. Commonly used guanidine salts include guanidine hydrochloride, guanidine isothiocyanate, etc. This salt can quickly cause the denaturation and lysis of the protein shell of the virus when the concentration is high, so that the viral nucleic acid in the preservation solution is released, and then it can be judged by nucleic acid detection Whether it is infected with a virus.
Desheng also has an inactivated sample transport media that does not contain guanidine salts, but uses other lysis salts, which have the same effect as guanidine salts, which are all lysis of virus proteins. There are also some preservation solutions that do not contain guanidine salts that can protect viral RNA and retain protein activity while inactivating the virus. This can be used for IgM, IgG antibody detection, and antigen detection in addition to nucleic acid detection.
Viruses can be detected after being inactivated by the Virus Transport Media because each cell or organism has a specific nucleic acid sequence, which can be determined by DNA amplification and sequencing. The nucleic acid of RNA virus needs to be converted into DNA by reverse transcription and then sequenced.
Southern blot hybridization is one of the important techniques for identifying specific DNA sequences in background genes. The principle is: two single DNA strands with certain homology can form double strands under certain conditions according to the principle of base complementation ( A=T, C=G). The two sides of the hybridization are the nucleic acid sequence to be tested and the labeled probe, and the hybridization process is highly specific.
The preservative reagent for virus inactivation is a new type of reagent derived from the original biochemical reagents by Desheng, and it is a necessary addition reagent in the virus sampling tube. Although it inactivates the virus, it retains the nucleic acid. Through RNA reverse transcription, DNA amplification and highly specific hybrid sequencing methods, nucleic acid detection can be performed quickly.
News
2023/09/27
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